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    Title: Production and purification of a fungal chitosanase and chitooligomers from Penicillium janthinellum D4 and discovery of the enzyme activators
    Authors: Anh Dzung Nguyen;Huang, Chun-Ching;Liang, Tzu-Wen;Van Bon Nguyen;Pan, Po-Shen;Wang, San-Lang
    Contributors: 淡江大學化學學系
    Keywords: Chitosanase;Squid pen;Penicillium janthinellum;Chitooligomers;Chitosanase activator;Boron
    Date: 2014-08-08
    Issue Date: 2014-08-08 14:59:41 (UTC+8)
    Publisher: Kidlington: Pergamon Press
    Abstract: Chitosanases have received much attention because of their wide range of applications. Although most fungal chitosanases use sugar as their major carbon source, in the present work, a chitosanase was induced from a squid pen powder (SPP)-containing Penicillium janthinellum D4 medium and purified by ammonium sulphate precipitation and combined column chromatography. The purified D4 chitosanase exhibited optimum activity at pH 7–9, 60 °C and was stable at pH 7–11, 25–50 °C. The D4 chitosanase that was used for chitooligomers preparation was studied. The enzyme products revealed various chitooligomers with different degrees of polymerisation (DP) from 3 to 9, as determined by a MALDI-TOF mass spectrometer, confirming the endo-type nature of the D4 chitosanase. D4 chitosanase activity was significantly inhibited by Cu2+, Mn2+, and EDTA. However, Fe2+ activated or inhibited D4 chitosanases at different concentrations. The D4 chitosanase was also activated by some small synthetic boron-containing molecules with boronate ester side chains.
    Relation: Carbohydrate Polymers 108, pp.331–337
    DOI: 10.1016/j.carbpol.2014.02.053
    Appears in Collections:[化學學系暨研究所] 期刊論文

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