English  |  正體中文  |  简体中文  |  全文筆數/總筆數 : 59061/92565 (64%)
造訪人次 : 730336      線上人數 : 36
RC Version 7.0 © Powered By DSPACE, MIT. Enhanced by NTU Library & TKU Library IR team.
搜尋範圍 查詢小技巧:
  • 您可在西文檢索詞彙前後加上"雙引號",以獲取較精準的檢索結果
  • 若欲以作者姓名搜尋,建議至進階搜尋限定作者欄位,可獲得較完整資料
  • 進階搜尋
    請使用永久網址來引用或連結此文件: http://tkuir.lib.tku.edu.tw:8080/dspace/handle/987654321/98310

    題名: Integrating SPR-ellipsometry and electrochemical measurements for performance evaluation of label-free thiophene-based biosensor
    作者: Tsai, Pei-I;Lee, Shu-Sheng;Chou, Shin-Ting;Chang, Yu-Ting;Lee, Adam Shih-Yuan;Lee, C.-K.
    貢獻者: 淡江大學化學學系;台灣大學應用力學研究所
    關鍵詞: Ellipsometry;Biosensors;Dielectric spectroscopy;Gold;Molecules;Phase shifts;Refractive index;Thin films;Biosensing
    日期: 2014-05
    上傳時間: 2014-07-11 12:18:57 (UTC+8)
    出版者: S P I E-International Society for Optical Engineering
    摘要: The surface plasmon resonance reflectance changes measured with a circularly polarized ellipsometry and an electrochemical impedance spectroscopy were identified to be able to characterize the critical roles of biomolecules for vastly different biological functions and processes. Throughout the course of this study, interferon-gamma (IFN-γ) was chosen as the biomarker to test and to verify the performance of this newly developed system for Tuberculosis detection. The interactions of IFN-γ with immobilized anti-IFN-γ antibody at various concentrations were interrogated both optically and electrochemically. A semi-conductive linker bis-thiophene was thiolated to ensure the cross-linked monoclonal human IFN-γ antibody got self-assembled onto the gold thin film and form a label-free biosensor. The functional features of the bis-thiophene coated-gold film were characterized by cyclic voltammetry and impedance spectroscopy methods. The association of IFN-γ to the bis-thiophene bridging units via antibody-antigen interactions provided the basis for ultrasensitive detection of IFN-γ by tracking the conformation changes in surface-bound protein molecules. The phase shift can be attributed to the average thickness and the real-time index of refraction of the protein layer in different protein layer. Experimental results obtained by impedance spectroscopy and by phase-interrogation SPR showed linear dynamic range. Our experimental results verified that an increase in the concentration of the IFN-γ usually accompanied by phase increase in SPR and an impedance decrease in EIS. These results indicated that our newly developed integrated biosensing system can potentially provide new insight into various conjugate phenomena and interfacial processes for observing molecular conformation changes.
    關聯: Proceedings of SPIE 8933, pp.89330H
    DOI: 10.1117/12.2038955
    顯示於類別:[化學學系暨研究所] 期刊論文


    檔案 描述 大小格式瀏覽次數



    DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library & TKU Library IR teams. Copyright ©   - 回饋