Liquid Crystal-based Immunoassay for Detecting Human Serum Albumin

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题名:	Liquid Crystal-based Immunoassay for Detecting Human Serum Albumin
作者:	陳志欣;何文薰
贡献者:	淡江大學化學學系
关键词:	Liquid Crystal-based Immunoassay
日期:	2013-11
上传时间:	2014-04-22 09:49:36 (UTC+8)
出版者:	Springer
摘要:	Human serum albumin (HSA) is the most abundant protein in human blood plasma. It is commonly used as a biomarker in urine samples to identify the chronic kidney disease (CKD) caused by high blood pressure or diabetes. In our research, a thin layer of liquid crystals (LCs) is used as a readout system for developing an immunoassay that reports the presence of HSA in the aqueous solution with optical signals. The detection principle of this assay is based on the variation of surface density of protein upon the specific binding of HSA on anti-HSA immobilized surface, which leads a dark-to-bright transition of LC images under cross polarizers. Our results show that the LC-based immunoassay can detect HSA at concentration of 50 ug/mL. By using the slide with immobilized anti-HSA in array format, the concentration level of HSA can be simply determined by the number of LC spot shown on the slide.
關聯:	The ﬁrst OPU–TKU International Symposium on Frontier Chemistry and Materials for the 21st Century
显示于类别:	[化學學系暨研究所] 會議論文

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