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http://tkuir.lib.tku.edu.tw:8080/dspace/handle/987654321/97767
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Title: | Low-potential amperometric determination of purine derivatives through surface oxide regeneration method |
Authors: | Chen, Chi-Hao;Lin, Ya-Ting;Lin, Meng-Shan |
Contributors: | 淡江大學化學學系 |
Keywords: | Uric acid;Xanthine;Hypoxanthine;Guanine;Adenine;Amperometry |
Date: | 2013-09-24 |
Issue Date: | 2014-04-21 09:58:09 (UTC+8) |
Publisher: | Amsterdam: Elsevier BV |
Abstract: | This article described a novel amperometry which can be used for determination of purine derivatives including uric acid, xanthine, hypoxanthine, guanine, and adenine without surface contamination. By applying a constant potential of −0.125 V (vs.Ag/AgCl) in a flow injection system, the chelating capability of these purine derivatives converts the cuprous oxide layer into a soluble complex. This behavior would dissolve the passive oxide layer and expose the bottom copper layer to the solution, subsequently; an oxidation current which attributed to the regeneration of the original cuprous oxide layer is used to reflect the concentration of these purine derivatives. In a 50 mM phosphate buffer, pH 7.0, this approach provides a high sensitivity with LOQ of sub-micro molar level of five purines and high stability with a RSD of 2.5% for 10 μM xanthine (N = 12). This method does not suffer from most biological species including ascorbic acid, acetaminophen, creatine, dopamine, sarcosine, ammonium ion, chloride ion, and urea at equal or higher than its physiological concentration. |
Relation: | Analytica Chimica Acta 796, pp.42–47 |
DOI: | 10.1016/j.aca.2013.08.011 |
Appears in Collections: | [化學學系暨研究所] 期刊論文
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1-s2.0-S0003267013010751-main.pdf | 期刊論文檔 | 1209Kb | Adobe PDF | 168 | View/Open | index.html | | 0Kb | HTML | 240 | View/Open |
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