English  |  正體中文  |  简体中文  |  Items with full text/Total items : 51931/87076 (60%)
Visitors : 8484972      Online Users : 181
RC Version 7.0 © Powered By DSPACE, MIT. Enhanced by NTU Library & TKU Library IR team.
Scope Tips:
  • please add "double quotation mark" for query phrases to get precise results
  • please goto advance search for comprehansive author search
  • Adv. Search
    HomeLoginUploadHelpAboutAdminister Goto mobile version
    Please use this identifier to cite or link to this item: http://tkuir.lib.tku.edu.tw:8080/dspace/handle/987654321/97764


    Title: Resveratrol treatment attenuates the wound-induced inflammation in zebrafish larvae through the suppression of myeloperoxidase expression.
    Authors: Liao, Y.F.;Chiou, M.C.;Tsai, J.N.;Wen, C.C.;Wang, Y.H.;Cheng, C.C.;Chen, Y.H.
    Contributors: 淡江大學數學學系暨研究所
    Keywords: cyclooxygenase;inflammation;myeloperoxidase;resveratrol;zebrafish
    Date: 2011-06
    Issue Date: 2014-04-21 09:03:01 (UTC+8)
    Publisher: Taipei: Bureau of Food and Drug Analysis
    Abstract: Resveratrol, a polyphenolic phytoalexin found in many plants, was reported to exhibit anti-inflammatory effects, but its molecular mechanism is not fully understood. This study was aimed to investigate the anti-inflammatory effects of resveratrol in vivo using a zebrafish model of wound-induced inflammation. Caudal fin-wounded zebrafish larvae were treated with resveratrol for 8 h. Neutrophil recruitment was visualized in transgenic line "Tg (mpx : GFP)" expressing GFP-tagged neutrophil-specific myeloperoxidase (mpx). The enzymatic activity of Mpx was evaluated by histochemical staining. Relative mRNA levels of mpx and cyclooxegenase-2 (cox2) were quantified by qRT-PCR, and the protein expression levels of Mpx and Cox2 were detected by immunostaining. Results showed that wound-induced neutrophil recruitment in zebrafish was not affected by resveratrol, but Mpx enzymatic activity in zebrafish was significantly suppressed by resveratrol in a dose-dependent manner. Moreover, both mRNA and protein expression levels of Mpx and Cox2 were significantly down-regulated by resveratrol. Taken together, our results provide in vivo evidence that the anti-inflammatory effects of resveratrol on wound-induced inflammation are significantly mediated through the suppression of Mpx and Cox2 at both transcriptional and protein levels, rather than the down-regulation of neutrophil recruitment. In conclusion, this in vivo zebrafish model can be readily applied to screen other potential anti-inflammatory compounds at a whole-organism level.
    Relation: Journal of Food and Drug Analysis 19(2), pp.167-173
    Appears in Collections:[數學學系暨研究所] 期刊論文

    Files in This Item:

    File Description SizeFormat
    index.html0KbHTML32View/Open
    PDF0KbHTML396View/Open

    All items in 機構典藏 are protected by copyright, with all rights reserved.


    DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library & TKU Library IR teams. Copyright ©   - Feedback