Fin reduction is a novel and unexpected teratogenic effect of amikacin-treated zebrafish embryos

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Title:	Fin reduction is a novel and unexpected teratogenic effect of amikacin-treated zebrafish embryos
Authors:	Chen, Y.H.;Tsai, I.T.;Wen, C.C.;Wang, Y.H.;Cheng, C.C.;Hu, S.C0;Chen, Y.H.
Contributors:	淡江大學數學學系暨研究所
Keywords:	Amikacin;fin;zebrafish;ototoxicity;neuromast
Date:	2012-01-15
Issue Date:	2014-04-21
Publisher:	New York: Informa Healthcare
Abstract:	We used zebrafish as a model to assess amikacin-induced embryotoxicity. We exposed zebrafish embryos to amikacin, using different amikacin doses (0-10 ppm), durations (12-48 h), and onsets (0, 24, 48 hpf). Amikacin-induced embryonic toxicity and reduced survival rate were found dependent on the exposure dose, duration and onset. Based on immunostaining with neuron-specific antibodies, amikacin reduced the number and size of zebrafish neuromasts. In addition, Amikacin caused pelvic, dorsal and anal fin defects in dose-dependent and duration-dependent manners. Proliferating cell nuclear antigen immunostaining revealed that amikacin-induced fin defects were not due to reduction of proliferating mesenchymal cells. TUNEL assay demonstrated that amikacin-induced fin defects might not associate with apoptosis. Therefore, further investigations are required to elucidate if other cell death pathways are involved in amikacin-induced fin defects.
Relation:	Toxicology Mechanisms and Methods 22(2), p.151-158
DOI:	10.3109/15376516.2011.610385
Appears in Collections:	[Graduate Institute & Department of Mathematics] Journal Article

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