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    Title: Tyrosinase inhibitors and insecticidal materials produced by Burkholderia cepacia using squid pen as the sole carbon and nitrogen source
    Authors: Hsua, Chia-Hao;Nguyenb, Anh Dzung;Chen, Yung-Wei;Wang, San-Lang
    Contributors: 淡江大學化學學系
    Keywords: Tyrosinase inhibitor;Squid pen;Insecticide;Burkholderia cepacia
    Date: 2014-07-01
    Issue Date: 2014-03-18 16:38:04 (UTC+8)
    Publisher: Dordrecht: Springer Netherlands
    Abstract: Reports of tyrosinase inhibitors from microorganisms are rare. A tyrosinase inhibitor- and insecticidal materials-producing bacterium, strain TKU026, was isolated from Taiwanese soil and identified as Burkholderia cepacia. Among the tested chitin-containing materials, squid pen best enhanced the production of tyrosinase inhibitors and insecticidal materials. The tyrosinase inhibitory activity (5000 U/mL) and insecticidal activity (81%) against Drosophila larvae was maximised after cultivation on 1% squid-pen containing medium for three days. The tyrosinase inhibitory activity persisted even when the culture was treated with acidic or alkaline conditions of pH 3 or 11. The activities of both tyrosinase inhibitors and insecticide remained at 100%, even after treatment at 100℃ for 30 min. The culture supernatant after three days of cultivation also showed antifungal activity against Aspergillus fumigatus and Fusarium oxysporum with maximal activities of 100% and 80%, respectively, but no antibacterial activity against Escherichia coli was observed. The tyrosinase inhibitors were assumed to be polyphenolic compounds according to the results of chromatography.
    Relation: Research on Chemical Intermediates 40(6), pp.2249-2258
    DOI: 10.1007/s11164-014-1602-0
    Appears in Collections:[化學學系暨研究所] 期刊論文

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