Production, purification and characterisation of a chitosanase from Bacillus cereus

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Title:	Production, purification and characterisation of a chitosanase from Bacillus cereus
Authors:	Wang, Chuan-Lu;Su, Jien-Wei;Liang, Tzu-Wen;Nguyen, Anh Dzung;Wang, San-Lang
Contributors:	淡江大學化學學系
Keywords:	Chitosanase;Squid pen;Bacillus cereus;Boric acid;Sodium tetraborate;Chitooligomers
Date:	2014-07
Issue Date:	2014-03-18 16:19:30 (UTC+8)
Publisher:	Dordrecht: Springer Netherlands
Abstract:	In the present work, a chitosanase was induced from a squid pen powder-containing Bacillus cereus TKU031 medium, and the addition of 0.05 % (w/v) boric acid or sodium tetraborate resulted in 195 and 177 % enhancement, respectively, in TKU031 chitosanase production. The purified TKU031 chitosanase exhibited optimum activity at pH 5 and 50 °C and was stable at pH 5–9 and <50 °C. The TKU031 chitosanase that was used for chitooligomers preparation was studied. The enzyme products revealed various chitooligomers with different degrees of polymerisation from 3 to 8, as determined by a MALDI-TOF–mass spectrometer, confirming the endo-type nature of the TKU031 chitosanase.
Relation:	Research on Chemical Intermediates 40(6), pp.2237-2248
DOI:	10.1007/s11164-014-1601-1
Appears in Collections:	[Graduate Institue of Life Sciences] Journal Article

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