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    題名: Gel electrophoresis of a charge-regulated, bi-functional particle
    作者: Hsu, Jyh-Ping;Huang, Chih-Hua;Tseng, Shiojenn
    貢獻者: 淡江大學數學學系
    關鍵詞: Brinkman fluid;Charge-regulated bi-functional particle;Double-layer polarization;Gel electrophoresis
    日期: 2013-03
    上傳時間: 2014-02-17 11:52:24 (UTC+8)
    出版者: Weinheim: Wiley - V C H Verlag GmbH & Co. KGaA
    摘要: Adopting a Brinkman fluid model, we analyzed the electrophoresis of a charged-regulated, bi-functional particle containing both acidic and basic functional groups in a gel solution. Both the long-range hydrodynamic effect arising from the liquid drag and the short-range steric effect from particle–polymer interaction are considered. The type of particle considered is capable of simulating both biocolloids such as microorganisms and cells, and particles with adsorbed polyelectrolyte or membrane layer. Our model describes successfully the experimental data in the literature. The presence of gel has the effect of reducing the particle mobility and alleviating double-layer polarization so that the particle behavior is less complicated than that in the case where gel is absent. On the other hand, both the quantitative and qualitative behaviors of a particle depend highly on solution pH and background salt concentration, yielding interesting and significant results. These results provide valuable information for both experimental data interpretation and electrophoresis devices design.
    關聯: Electrophoresis 34(5), pp.785–791
    DOI: 10.1002/elps.201200370
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