聚羥基烷酯類[poly(hydroxyalkanoate)，PHA]，是微生物在其細胞中自行合成的一種聚酯類高分子，本實驗中採用不同的提取方法，將高分子由細胞中提取分離出來，探討分離效率及PHA產物結構。所採用的方法有：索氏萃取法、直接萃取法、氯仿/雙氧水處理法、氯仿/去離子處理法、界面活性劑法。經由這些方法所取得的PHA高分子利用FTIR、NMR等儀器來分析結構，GPC儀器測量PHA的分子量。最後比較不同的提取方法的優缺點及PHA的純度。 Poly(hydroxyalkanoate), PHA is a polyester produced from the bacterial fermentation. In this research, different methods were employed to extract the produced PHA from bacterial cell. These methods include Soxlet extraction, direct solvent extraction, chloroform/H2O2 treatment ,chloroform/H2O treatment, and surfactant treatment method. FTIR and NMR were used to analyze the structures of the recovery PHA. The molecular weight of PHA was measured by GPC. The effects of different extraction methods on the molecular weight and properties of PHA were thus discussed and compared.
