本研究選用無機奈米薄膜，以連續式掃流過濾系統對蛋白質溶液進行過濾實驗，探討不同操作條件及溶液性質對濾速與薄膜結垢的影響，以尋求提升濾速之操作方式。首就蛋白質溶液於管式單通道無機薄膜過濾系統之濾速行為，討論操作參數(透膜壓差、濃度、pH值、掃流速度等)對濾速之影響，並定量薄膜結垢之阻力，找出適當之清洗方法。 結果顯示，NaOH溶液可有效清洗移除附著於膜上BSA之結垢，但隨著溶液濃度之增加也需提高NaOH清洗液之濃度。當操作流態為亂流時，BSA進料濃度對濾速之影響不大。在BSA與β-cyclodextrin的混合溶液系統中，薄膜對β-cyclodextrin之阻隔率隨溶液pH值不同而有所改變。以5k Da薄膜為例，當pH=6.87時，β-cyclodextrin之阻隔率高達80%，而當pH=10時，則有低於30%之阻隔率，這是因為BSA粒子形成之極化層緊密度不同所致。因此，選擇適當pH值可將BSA溶液中之小分子進行分離。 In this study, the inorganic tubular membranes (1000, 5000 MWCO) were employed in a cross-flow filtration system to investigate the effects of operation conditions, solutions and membrane properties on protein solutions filtration. The ways for cleaning the membrane fouling were also discussed. The solution fluxes and solute rejections were measured under various operating parameters such as membrane MWCO, transmembrane pressure, pH value and solution composition. Experimental results indicate that the NaOH solution could remove the BAS fouling on the membrane and the required concentration of NaOH solution increases as the feed BSA concentration increases. Under the turbulent flow pattern, the increase in BSA concentration just slightly reduces the flux. For BSA and beta-cyclodextrin binary solution, the rejection of beta-cyclodextrin varies with the pH value. In the case of 5k Da membrane, the rejection of beta-cyclodextrin is higher than 80% at pH 6.87 and less than 30% at pH 10. This is due to the fact that the porosity of the polarization layer of BSA on the membrane surface varies with pH value. Therefore, the present membrane can be applied for the separation of small molecule from BSA solution by choosing a suitable pH value.
