| 摘要: | 本研究以相轉換法製備生物分解性高分子－聚丁基烷酯Poly(3-hydroxybutyrate)薄膜，並將其表面改質，接枝聚丙烯酸及固定離胺酸，然後進行骨母細胞(hFOB 1.19)培養以探討其生物相容性。薄膜之孔隙結構與孔隙尺寸可藉由製膜參數(例如製膜液溶解溫度)加以控制，表面孔徑約在數微米範圍(參閱92年成果報告)；接枝聚丙烯酸乃以電漿聚合法進行，實驗結果顯示接枝條件如電漿處理時間、電漿功率大小、接枝反應時間、單體濃度及薄膜結構等皆可影響接枝量(參閱92年成果報告)，本研究最大接枝量可達0.48 mg/cm2；固定離胺酸乃利用1-ethyl-3-(3-diethylaminopropyl) carbodiimide hydrochloride (EDAC)為媒介使離胺酸與丙烯酸反應形成醯胺鍵，而將離胺酸固定於薄膜上，固定量受反應條件如EDAC濃度、反應pH值、反應時間、離胺酸濃度、薄膜孔隙結構等影響，最大固定量為0.55 g/cm2。各薄膜皆用於骨母細胞培養，結果顯示細胞在多孔型薄膜上之貼附與生長情形優於緻密型薄膜，聚丙烯酸會抑制細胞之成長，而離胺酸則可提升，然而後者之固定量太低，所以提升成效受到限制。
In this research, membranes of a biodegradable polymer, poly(3-hydroxybutyrate), were synthesized by the phase inversion technique. The surfaces of the formed membranes were modified by first grafting with poly(acrylic acid) and then immobilizing with lysine. These membranes were employed in culture of osteoblast (hFOB 1.19) to examine their biocompatibility. The porous structure and pore size of the membrane were controlled by formation parameters, e.g., dissolution temperature of casting dope. The pore size of the surface fell on the range of several microns. Plasma-induced polymerization method was used to graft poly(acrylic acid) on the membrane surface. Various process variables, viz., plasma treatment time, plasma power, grating reaction time, monomer concentration, and membrane porous structure were found to affect the graft yield, for which the largest attainable value was 0.48 mg/cm2 under optimal conditions. Immobilization of lysine on the membranes was carried out by forming amide bonds with previously grafted poly(acrylic acid), using 1-ethyl-3-(3-diethylaminopropyl) carbodiimide hydrochloride (EDAC) as a medium. The immobilization yields were dependent on reaction conditions, such as concentration of EDAC, pH, reaction time, concentration of lysine, membrane structure, etc., with a highest yield of 0.55 g/cm2. Culture of osteoblast cell on various membranes indicated a better attachment and growth of the cell on porous membranes than on dense ones. Poly(acrylic acid) inhibited the growth of osteoblast cell, while, by contrast, lysine promoted it. However, as a result of low immobilization yield, the latter improvement was confined. Keywords: biodegradable polymer, poly(3-hydroxybutyrate), porous membrane, surface modification, lysine, cell culture. |
