本實驗採用一透析過濾裝置進行實驗，以單成分HA溶液、單成分LY溶液以及HA/LY雙成分溶液為實驗對象，並採用批次恆壓型式，針對攪拌速度、電解質種類、電解質濃度、透析液體積、透析液電解質濃度等操作變數之改變進行實驗分析。 研究結果顯示，加入攪拌可有效降低過濾阻力而提高濾速，電解質離子強度越大，使玻尿酸分子萎縮捲曲的程度越大，使得HA堆積形態越為緊密，濾速相對較低；而電解質造成LY與薄膜以及HA之間的靜電作用力降低，使LY不易吸附於膜面上，較易通過薄膜，提高移除效率。隨著透析過濾操作之進行，增加透析體積有助於批次濾出進料槽中之LY；若透析液中電解質濃增加有助於LY之移除率，但到達一定濃度後，再增加電解質濃度反而會影響LY的移除。 In this study, the HA solution, LY solution and the mixture solution of HA/LY were used as the feed solution, and experimented by adopting diafiltration in a dead-end stirred cell. The experimented results were discussed under different operating conditions such as stirred rate, electrolyte type, electrolyte concentration and diavolumes. The results shows that the addition of stirring can reduce the filtration resistance and improve the permeate flux. The permeate flux decreases with the increase of ion strength of electrolyte which causes the HA molecules become more shrunken and then form a compact deposited layer on membrane surface. However, the LY molecules can pass through the membrane more easily as the electrolyte is added. The strength of electrode ion will hinder the surface charge of LY, and decrease the electrostatic interference between LY molecules and membrane. During the diafiltration process of HA/LY solution, the diavolumes and electrolyte concentration can increase the removal of LY. But there is a limit to the increase of electrolyte concentration in dialyate solution, instead, would decrease the removal of LY.