English  |  正體中文  |  简体中文  |  Items with full text/Total items : 51296/86412 (59%)
Visitors : 8173976      Online Users : 90
RC Version 7.0 © Powered By DSPACE, MIT. Enhanced by NTU Library & TKU Library IR team.
Scope Tips:
  • please add "double quotation mark" for query phrases to get precise results
  • please goto advance search for comprehansive author search
  • Adv. Search
    HomeLoginUploadHelpAboutAdminister Goto mobile version
    Please use this identifier to cite or link to this item: http://tkuir.lib.tku.edu.tw:8080/dspace/handle/987654321/93997


    Title: Penicillium janthinellum D4所生產幾丁聚醣酶之純化與定性
    Other Titles: Purification and characterization of chitosanase from Penicillium janthinellum D4
    Authors: 黃淳靖;Huang, Chun-Ching
    Contributors: 淡江大學化學學系碩士班
    王三郎;Wang, San-Lang
    Keywords: 幾丁聚醣酶;烏賊軟骨;抑制劑;Penicillium
    Date: 2013
    Issue Date: 2014-01-23 13:46:23 (UTC+8)
    Abstract: Penicillium janthinellum D4株篩選自越南Tay Nguyen大學之幾丁聚醣酶生

    產菌。本研究探討P. janthinellum D4發酵烏賊軟骨生產幾丁聚醣酶之較適條

    件,所得發酵上清液經硫酸銨沉澱、DEAE-Sepharose離子交換層析、

    Macro-prep DEAE Cartridge等步驟,純化出兩種幾丁聚醣酶F1及F2。幾丁聚

    醣酶F2之分子量經SDS-PAGE測量為49kDa,其最適反應溫度、最適反應

    pH、熱安定性以及pH安定性分別為60℃、pH 7~9、25~40℃以及pH 10。於

    抑制劑方面,幾丁聚醣酶F2的活性會受Fe2+、Cu2+、Mn2+所抑制。
    The chitosanase producing strain Penicillium janthinellum D4, was

    isolated from soil in Tay Nguyen University in Vietnam. Two

    chitosanases F1 F2 were purified from the culture supernatant by

    ammonium sulfate precipitation, DEAE-Sepharose column

    chromatography and Macro-prep DEAE Cartridge. The molecular mass

    of CHS was estimated to be 49kDa by SDS-PAGE. The optimum

    temperature, optimum pH, thermal stability, and pH stability of CHS F2

    were 60℃, pH 7~9, 25~40℃, and pH 10, respectively. The activity of CHS F2 was

    inhibited by Fe2+,Cu2+,and Mn2+.
    Appears in Collections:[化學學系暨研究所] 學位論文

    Files in This Item:

    File SizeFormat
    index.html0KbHTML148View/Open

    All items in 機構典藏 are protected by copyright, with all rights reserved.


    DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library & TKU Library IR teams. Copyright ©   - Feedback