English  |  正體中文  |  简体中文  |  全文筆數/總筆數 : 60917/93528 (65%)
造訪人次 : 1560859      線上人數 : 15
RC Version 7.0 © Powered By DSPACE, MIT. Enhanced by NTU Library & TKU Library IR team.
搜尋範圍 查詢小技巧:
  • 您可在西文檢索詞彙前後加上"雙引號",以獲取較精準的檢索結果
  • 若欲以作者姓名搜尋,建議至進階搜尋限定作者欄位,可獲得較完整資料
  • 進階搜尋
    請使用永久網址來引用或連結此文件: https://tkuir.lib.tku.edu.tw/dspace/handle/987654321/93990


    題名: Bacillus cereus TKU033所生產幾丁聚醣酶之純化及其應用
    其他題名: Purification and characterization of chitosanase from Bacillus cereus TKU033 and it's application
    作者: 黃稚婷;Huang, Chih-Ting
    貢獻者: 淡江大學化學學系碩士班
    王三郎;Wang, San-Lang
    關鍵詞: Bacillus cereus;幾丁聚醣酶;烏賊軟骨;chitosanase;squid pen powder
    日期: 2013
    上傳時間: 2014-01-23 13:46:01 (UTC+8)
    摘要: 菌株 TKU033 係以烏賊軟骨為唯一碳/氮源,篩選至台灣三芝土壤經鑑定為 Bacillus cereus之幾丁聚醣酶生產菌。幾丁聚醣酶之較適培養條件為,以含有1.5% 烏賊軟骨粉、0.1%K2HPO4、0.05%MgSO4.7H2O之50mL液態培養基於 37℃搖瓶(150 rpm)培養2天,所得發酵上清液經硫酸銨沉澱、DEAE-Sepharose 及Macro-Prep DEAE Cartidge離子交換樹脂等步驟,純化出一種幾丁聚醣酶。此幾丁聚醣酶之分子量經SDS-PAGE測為43 kDa ,最適反應pH、最適溫度、pH安定性及熱安定性分別為 pH 5、50℃、pH5-7及<40℃,其活性會受 Cu2+ 、Mn2+ 和EDTA所抑制。
    較適培養條件所製得B.cereus TKU033粗酵素液,幾丁聚醣酶水解水溶性幾丁聚醣,所得上清液添加於乳酸菌Lactobacillus paracasei 12193及 Lactobacillus kefri 14011的MRS液態培養基,可分別促進乳酸菌生長達141%及116% 之影響。
    The chitosanase-producing strain TKU033 was isolated from soil in Taiwan, Sanzhi with squid pen powder as the sole carbon/nitrogen source and identified as Bacillus cereus. A chitosanase was purified from the culture supernatant by ammonium sulfate precipitation, DEAE-Sepharose and Macro-Prep DEAE Cartridge. The molecular mass of chitosanase determined by SDS-PAGE was approximately 43 kDa. The optimun pH , optimun temperature, pH stability, and thermal stability of chitosanase were pH 5,
    50℃, pH5-7 and <40℃. The chitosanase activity was inhibited by Cu2+ , Mn2+ and EDTA.
    The supernatant of the crude enzyme from B. cereus TKU033 hydrolyzed water soluble chitosan exhibited activity of enhancing growth for L. paracasei 12193 up to 141% and L.kefri 14011 up to 116%.
    DOI: 10.6846%2fTKU.2013.00934
    顯示於類別:[化學學系暨研究所] 學位論文

    文件中的檔案:

    檔案 描述 大小格式瀏覽次數
    index.html0KbHTML413檢視/開啟
    index.html0KbHTML115檢視/開啟

    在機構典藏中所有的資料項目都受到原著作權保護.

    TAIR相關文章

    DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library & TKU Library IR teams. Copyright ©   - 回饋