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    Title: 啤酒酵母醛類去氫酶2和3,紫外光及過氧化物的影響
    Other Titles: Effects of ultraviolet and peroxide on aldehyde dehydrogenase 2 and 3 from saccharomyces cerevisiae
    Authors: 曾昱縉;Zeng, Yu-Jin
    Contributors: 淡江大學化學學系碩士班
    簡素芳;陳銘凱;Chien, Su-Fang;Chern, Ming-Kai
    Keywords: 醛類去氫酶2;醛類去氫酶3;啤酒酵母菌;aldehyde dehydrogenase 2;aldehyde dehydrogenase 3;Saccharomyces cerevisiae
    Date: 2013
    Issue Date: 2014-01-23 13:45:39 (UTC+8)
    Abstract: 啤酒去氫酶 ALD2p 和 ALD3p 為 ACDH (acetaldehyde dehyrogenase) 家族中的成員,並利用 NAD+ 作為輔酶。文獻指出,在泛酸合成的途徑中, ALD2p 和 ALD3p 會將中間產物 3-aminopropanal 轉變成為 β-alanine。

    在哺乳類中, ALDHs 存在於角膜蛋白質中,以保護眼睛,透過直接吸收紫外光、代謝脂質過氧化物、抗氧化的功能保護眼睛。

    由於先前實驗測試不到 ALD2p 及 ALD3p 的活性,所以在此測試是否有其他的功能,例如上述的抗紫外光、代謝脂質過氧化物、抗氧化的功能。

    透過剔除 ALD2、ALD3 基因及過度表現 ALD2、ALD3 基因,接著再用滅菌過的超純水清洗,稀釋成不同濃度將菌液沾點於固態培養基上,照射紫外光或稀釋成不同濃度沾點於含有 H2O2 的固態培養基上。
    In Saccharomyces cerevisia ALD2 (YMR170c) and ALD3 (YMR169c) are the members of ACDH (acetaldydehyde dehydrogenase) family, and the gene products are supposed to use NAD+ as coenzyme. Recent reference also shows that ALD2 and ALD3 genes are require for pantothenic acid biosynthesis by conversion of 3-aminopropanal to β-alanine.

    In mammals, ALDHs have been identified as corneal crystallins and to contribute to eye protection by directly absorbing ultraviolet, or detoxifying reactive aldehydes produced by lipid peroxidation to non-toxic metabolities and act as antioxidant.

    In this study, we examined the effects of ultraviolet and peroxide by deletion and overexpression of ALD2 and ALD3 gene.
    Appears in Collections:[化學學系暨研究所] 學位論文

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