透過剔除 ALD2、ALD3 基因及過度表現 ALD2、ALD3 基因，接著再用滅菌過的超純水清洗，稀釋成不同濃度將菌液沾點於固態培養基上，照射紫外光或稀釋成不同濃度沾點於含有 H2O2 的固態培養基上。 In Saccharomyces cerevisia ALD2 (YMR170c) and ALD3 (YMR169c) are the members of ACDH (acetaldydehyde dehydrogenase) family, and the gene products are supposed to use NAD+ as coenzyme. Recent reference also shows that ALD2 and ALD3 genes are require for pantothenic acid biosynthesis by conversion of 3-aminopropanal to β-alanine.
In mammals, ALDHs have been identified as corneal crystallins and to contribute to eye protection by directly absorbing ultraviolet, or detoxifying reactive aldehydes produced by lipid peroxidation to non-toxic metabolities and act as antioxidant.
In this study, we examined the effects of ultraviolet and peroxide by deletion and overexpression of ALD2 and ALD3 gene.
