淡江大學機構典藏:Item 987654321/93053
English  |  正體中文  |  简体中文  |  Items with full text/Total items : 62797/95867 (66%)
Visitors : 3732028      Online Users : 462
RC Version 7.0 © Powered By DSPACE, MIT. Enhanced by NTU Library & TKU Library IR team.
Scope Tips:
  • please add "double quotation mark" for query phrases to get precise results
  • please goto advance search for comprehansive author search
  • Adv. Search
    HomeLoginUploadHelpAboutAdminister Goto mobile version
    Please use this identifier to cite or link to this item: https://tkuir.lib.tku.edu.tw/dspace/handle/987654321/93053


    Title: Purification of chitinase/chitosanase from Bacillus cereus and discovery of an enzyme inhibitor
    Authors: Liang, Tzu-Wen;Chen, Yue-Yin;Pan, Po-Shen;Wang, San-Lang
    Contributors: 淡江大學化學學系
    Keywords: Bacillus cereus;Boron;Chitinase;Chitinase inhibitor;Chitosanase;Squid pen
    Date: 2013-10-26
    Issue Date: 2013-11-12 12:13:24 (UTC+8)
    Publisher: Amsterdam: Elsevier BV
    Abstract: A chitinase and a chitosanase were induced from a squid pen powder (SPP)-containing medium of Bacillus cereus TKU030 and purified by precipitation with ammonium sulphate and combined column chromatography. The purified chitinase and chitosanase exhibited optimum activity at 60 °C, pH 5–6 and 40 °C, pH 4, respectively. The chitinase and chitosanase were stable at 25–60 °C, pH 4–7 and 25–50 °C, pH 3–7, respectively. The chitinase and chitosanase showed the highest activity toward β-chitin and 60% DD chitosan, respectively. The chitinase was significantly inhibited by Mn2+ and EDTA but activated by Cu2+, Fe2+ and Ca2+. The chitosanase was significantly inhibited by Cu2+, Fe2+, Zn2+, Mn2+ and EDTA. The chitinase showed high stability in the presence of various surfactants, such as SDS, Tween 20, Tween 40 and Triton X-100. In contrast, these surfactants were inhibitors of the chitosanase. The chitinase and chitosanase were also inhibited by TKUPSP017, a small synthetic boron-containing molecule with a BF3K side-chain. However, TKUPSP017 enhanced the growth of B. cereus TKU030 in SPP-containing medium.
    Relation: International Journal of Biological Macromolecules 63, pp.8-14
    DOI: 10.1016/j.ijbiomac.2013.10.027
    Appears in Collections:[Graduate Institute & Department of Chemistry] Journal Article

    Files in This Item:

    File Description SizeFormat
    index.html0KbHTML454View/Open
    JBIOMAC TKU030.pdf954KbAdobe PDF852View/Open

    All items in 機構典藏 are protected by copyright, with all rights reserved.


    DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library & TKU Library IR teams. Copyright ©   - Feedback