Amikacin-induced Fin Reduction is Mediated by Autophagy

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Title:	Amikacin-induced Fin Reduction is Mediated by Autophagy
Authors:	Tsai, I-Ting;Chen, Yau-Hung;Wang, Yun-Hsin
Contributors:	淡江大學生命科學研究所
Keywords:	amikacin;autophagy;embryotoxicity;fin reduction;zebrafish
Date:	2013-03-01
Issue Date:	2013-07-29 09:22:45 (UTC+8)
Publisher:	Tokyo: Japanese Society of Toxicologic Pathology
Abstract:	Despite its medical use, little is known about the mechanisms underlying amikacin-induced embryotoxicity, including fin reduction, in zebrafish. In this study, we examined the expression of well-known autophagy markers mTOR (target of rapamycin), atg10 (autophagy-related gene), atg12 and LC3 (mammalian homolog of Atg8) in amikacin-treated zebrafish embryos. Our results indicated that the mRNA expression level of atg12 in the amikacin-treated group was significantly increased by 1.5-fold (p＜0.05) compared with the corresponding mock control group, while the expression levels of atg10 and mTOR were significantly decreased by 0.74-fold (p＜0.05) and 0.58-fold (p＜0.05), respectively. Western blot analysis revealed that LC3 protein expression was induced by amikacin. Taken together, these data suggest that amikacin-induced fin reduction is mediated by fin cell autophagy.
Relation:	Journal of Toxicologic Pathology 26(1), pp.79-82
DOI:	10.1293/tox.26.79
Appears in Collections:	[Graduate Institute & Department of Chemistry] Journal Article

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