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    Please use this identifier to cite or link to this item: http://tkuir.lib.tku.edu.tw:8080/dspace/handle/987654321/89108

    Title: A Liquid Crystal Biosensor for Detecting Organophosphates through the Localized pH Changes Induced by Their Hydrolytic Products
    Authors: Chen, Chih-hsin;Yang, Kun-lin
    Contributors: 淡江大學化學學系
    Keywords: Organophosphate;Biosensors;Liquid crystal;Paraoxonase 1
    Date: 2013-05-01
    Issue Date: 2013-05-14 13:36:33 (UTC+8)
    Publisher: Elsevier BV
    Abstract: We report a biosensor for detecting organophosphates (OPs) by using liquid crystal (LC). The mechanism of the biosensor is based on detection of minute pH changes during the enzymatic hydrolysis of OPs. To hydrolyze OPs, paraoxonase 1 (PON1) is immobilized on a copper grid which also hosts pH-sensitive LC, 4-cyano-4′-pentylbiphenyl (5CB) doped with 0.3% of 4′-pentyl-biphenyl-4-carboxylic acid (PBA). Proximity of enzyme and LC ensures that H+ can be detected by the pH-sensitive LC before it is neutralized by buffer. Thus, the presence of OPs can be reported as color change in LC when they undergo enzymatic hydrolysis. For paraoxon detection, we can reach a limit of detection (LOD) of 1 μM by using the naked eye. This type of LC-based biosensor also shows high specificity and does not respond to imidacloprid and ampicillin.
    Relation: Sensors and Actuators B: Chemical 181, pp.368-374
    DOI: 10.1016/j.snb.2013.01.036
    Appears in Collections:[Graduate Institute & Department of Chemistry] Journal Article

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