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    Please use this identifier to cite or link to this item: http://tkuir.lib.tku.edu.tw:8080/dspace/handle/987654321/88044


    Title: 微流道表粗對於細胞貼附之影響
    Other Titles: Surface roughness effect on the cell attachment in a microchannel
    Authors: 呂傑文;Lu, Chieh-Wen
    Contributors: 淡江大學機械與機電工程學系碩士班
    楊龍杰;Yang, Lung-Jieh
    Keywords: 微流道;表面粗糙度;細胞貼附;Microfluidic;PDMS;surface roughness;Cell attachment
    Date: 2012
    Issue Date: 2013-04-13 11:58:13 (UTC+8)
    Abstract: 本研究以黃光微影技術和軟微影PDMS 翻模技術,製作出一個仿微血管
    水力直徑的PDMS 微流道。先行驗證PDMS 在O2 與CF4 混合氣體不同比例下,
    電漿蝕刻的表面粗糙度變化數據,再利用該不同表面粗糙度的PDMS 微流道,
    透過微球體與活體肝癌細胞HepG2 之動態灌流實驗,分別觀察二者貼附於
    PDMS 微流道之每隔10 分鐘變動的面積多寡,而至終定性歸納出,PDMS 微
    流道表粗對於細胞貼附之影響。
    A PDMS microchannel mimicking the capillary blood vessel is fabricated by the soft
    lithography. With treating the PDMS microchannels by different plasmas, the
    corresponding surface roughness RA data are experimentally measured. Using these PDMS
    microchannels well defined by proper RA values, liquid streams with microbeads and
    living HepG2 tumor cells are filled in, respectively. The microbead and cell attachment
    areas in the PDMS microchannel have been recorded dynamically with 10 min interval.
    Finally, the author qualitatively discusses the surface roughness effect on the particle or
    cell attachment in a PDMS microchannel.
    Appears in Collections:[機械與機電工程學系暨研究所] 學位論文

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