English  |  正體中文  |  简体中文  |  Items with full text/Total items : 51317/86412 (59%)
Visitors : 8179263      Online Users : 78
RC Version 7.0 © Powered By DSPACE, MIT. Enhanced by NTU Library & TKU Library IR team.
Scope Tips:
  • please add "double quotation mark" for query phrases to get precise results
  • please goto advance search for comprehansive author search
  • Adv. Search
    HomeLoginUploadHelpAboutAdminister Goto mobile version
    Please use this identifier to cite or link to this item: http://tkuir.lib.tku.edu.tw:8080/dspace/handle/987654321/87430


    Title: 含硼之胜肽衍生物合成與其生物活性之評估
    Other Titles: Synthesis and biological evaluation of boron-containing peptide-based analogues
    Authors: 謝青典;Hsieh, Ching-Tien
    Contributors: 淡江大學化學學系碩士班
    潘伯申
    Keywords: 含硼胜肽化合物;抗癌;斑馬魚;boron;peptide;anti-cancer;Zebrafish
    Date: 2012
    Issue Date: 2013-04-13 11:04:49 (UTC+8)
    Abstract: 本論文設計與合成了兩系列含硼胜肽化合物。此兩類化合物可以以簡單的合成方法製備與純化。系列A:胜肽的起始物與胜肽化合物經偶和反應後生成硼酯胜肽化合物。硼酯胜肽化合物可利用KHF2水溶液將硼酯轉換成三氟硼酸化合物。三氟硼酸化合物可利用SiO2在EtOAc/H2O = 1:1之溶液中輕易地將三氟硼酸轉變成相對應的硼酸化合物;系列B:胜肽化合物與含三氟硼酸官能基的起始物在甲醇反應形成imine中間物後,加入PEMB以形成三氟硼酸基之類胜肽化合物。
    在抑制MDA-MB231(乳癌細胞)以及HepG2(肝癌細胞)的實驗中,藉由改變胜肽化合物的結構與含硼官能基來觀察結構之改變與抑制MDA-MB231、HepG2的關係。實驗結果發現,系列A:化合物A-07、A-08、A-09、A-11、A-12對於HepG2癌細胞具有抑制的效果。有趣的是,增加胜肽結構之長度並不會對抗癌活性有所幫助,而是α位置上的取代基對於抑制效果具有重要的影響,另外,在硼官能基中則是以三氟硼酸的抑制效果最好。系列B:僅有部分三胜肽含硼化合物對於MDA-MB231、HepG2有抑制效果。其最佳結構條件為:第一號位置為Val,第二號位置必須為Phe,第三號位置須為Phe (B38)或者是Ser(Bzl) (B41),而硼的官能基則是以三氟硼酸的表現較好。
    In the thesis, we have designed and synthesized two series of boron-containing peptide analogues. These analogues were prepared via efficient and straightforward synthetic protocols. Biological evaluations of synthesized compounds were accomplished through zebra fish and MTT assays respectively.
    Structure-activity relationship (SAR) indicated that A-07, A-08, A-09, A-11 A-12 are potent against HepG2 (liver cancer) cells but not MDA-MB231 (drug resistant breast cancer) cells. B38, were found potent against HepG2 but not MDA-MB231. B41 being one of the most potent analogues of 2 series, was active against HepG2 and MDA-MB231 cell lines. In summary, inclusion of a phenylalanine at position 2, a benzyl-protected serine at position 3, and a potassium trifluoroborate greatly improved the potency of the molecule against either HepG2 or MDA-MB231 cell lines.
    Appears in Collections:[化學學系暨研究所] 學位論文

    Files in This Item:

    File SizeFormat
    index.html0KbHTML90View/Open

    All items in 機構典藏 are protected by copyright, with all rights reserved.


    DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library & TKU Library IR teams. Copyright ©   - Feedback