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    Please use this identifier to cite or link to this item: http://tkuir.lib.tku.edu.tw:8080/dspace/handle/987654321/87409

    Title: 幾丁類物質於色素吸附之利用
    Other Titles: Utilization of chitinous materials on pigments adsorption
    Authors: 陳彥丞;Chen, Yan-Cheng
    Contributors: 淡江大學化學學系碩士班
    Keywords: 靈菌紅素;沙雷氏菌;乳酸桿菌;幾丁質;食用色素;prodigiosin;Serratia marcescens;Lactobacillus;chitin;food colorant
    Date: 2012
    Issue Date: 2013-04-13 11:03:38 (UTC+8)
    Abstract: 靈菌紅素(prodigiosin;PG)具有諸如抗癌、抗菌以及殺蟲等生物活性。本研究針對Serratia marcescens TKU011發酵烏賊軟骨生產靈菌紅素之條件進行探討。結果發現磷酸鹽會抑制靈菌紅素的生產。此1.5%烏賊軟骨培養基經高壓滅菌後,添加50mL蒸餾水於離心所得沉澱物作為培養基,於30℃發酵三天所得靈菌紅素產量 (2.38mg/mL)提升約2.5倍。
    Prodigiosins are known to exert antitumor, antibacterial and insecticidal activities. Using fishery-processing wastes of squid pen power(SPP)as the sole carbon and nitrogen(C/N)source, Serratia marcescens TKU011 produced prodigiosin. The relationship between prodigiosin production and phosphate concentration was studied. The medium(1.5%SPP、0.05%MgSO4‧7H2O、0.1%K2HPO4) was sterilized and centrifuged, and the deposits were collected and supplied 50ml water. Production in the medium at 30 ℃ for 3 days showed highest level of prodigiosin production(2.38mg/mL).
    Lactic acid bacteria, cotton, and Bombax ceiba have been tested for their enhancing effects on prodigiosin production of S. marcescensTKU011 by supplementing the cells and adsorbents into SPP-containing medium. Bombax ceiba showed best results of increasing 5-folds of prodigiosin productivity compared with the non-supplement group. Among SPP and other chitinous materials of cicada casting powder(CCP), shrimp shell power(SSP)and chitin, lactic acid bacteria and CCP showed best adsorption on prodigiosin.
    As for the adsorbing effects of water-soluble food colorants of allura red AC(R40)and tartrazne(Y4),SPP showed the best adsorption. The capacities of SPP adsorption for R40 and Y4 were determined to be 14mg/g and 12.3 mg/g respectively. Regeneration experiments showed that the water-soluble pigments can be completely regenerated with surfactant-SDS solution.
    Appears in Collections:[Graduate Institute & Department of Chemistry] Thesis

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