|Abstract: ||本研究主要探討聚偏二氟乙烯(PVDF)多孔型薄膜之製備、改質及其在生醫技術方面的運用。首先利用浸漬-沈澱法製備聚偏二氟乙烯(PVDF)薄膜，溶劑為磷酸三乙酯(TEP)，非溶劑為水；藉由改變製膜液與沈澱槽之組成，製作出一系列不同孔隙結構之薄膜，並以SEM、DSC、XRD、Contact Angle等來作膜材物性分析。其次，利用電漿聚合法將聚甲基丙烯酯環氧丙烷(PGMA)接枝在各薄膜上，並探討電漿處理時間、電漿功率、薄膜孔隙結構、反應溫度、反應濃度等參數對接枝量的影響，於多孔型薄膜有最大接枝量0.293 mg/cmP2P；再利用PGMA的環氧基分別與離胺酸及己二胺上的胺基反應形成共價鍵，而將此二者固定於薄膜表面，同樣地對各反應參數(例如反應溫度、反應濃度、pH等)加以探討，於多孔型薄膜有最高固定離胺酸的量0.278 mg/cmP2P，最高固定己二胺的量為0.222 mg/cmP2P。最後將各種不同孔隙結構或化學組成的薄膜應用於神經細胞的培養，結果發現離胺酸與己二胺化學組成上唯一的差異-酸基，對於神經細胞的培養有相當程度的影響。|
This research investigate the preparation, modification, and application in the biomedical field of porous poly(vinylidene fluoride) (PVDF) membranes. PVDF membranes with different porous structures were prepared by immersion-precipitation of a casting solution in coagulation baths composed of triethylphosphate (TEP, solvent) and water (nonsolvent). SEM, DSC, XRD, and Contact Angle analyzer were used to characterize the membranes. Onto the membrane surface, an amino acid, l-lysine, and 1,6-hexanediamine were immobilized by a chemical process. First, the membrane was grafted with poly(glycidyl methacrylate) (PGMA) by means of plasma-induced free radical polymerization. Then, lysine and 1,6-hexanediamine were immobilized on the membranes by reaction of them with epoxy group of previously grafted PGMA. The highest attainable grafting yield of PGMA on PVDF membrane reached up to 0.293 mg/cmP2P. For immobilization of l-lysine and 1,6-hexanediamine on the membrane, the yields were found to depend on factors, such as reaction concentrations, reaction temperature, and pH value. The maximal attainable immobilization yields were 0.278 mg/cmP2P and 0.222 mg/cmP2P, respectively. Furthermore, neuron cells were cultured on l-lysine/PGMA/PVDF and 1,6-hexanediamine/PGMA/PVDF membranes. It was found that chemical compositions on the membrane, in particular, the carboxylic acid of l-lysine, had a marked influence on the cell activity.