Bacillus subtilis W-118, an antifungal materials producing strain, excreted one chitinase when cultured in a medium containing shrimp and crab shell powder as major carbon source. The chitinase, purified by sequential chromatography, had a molecular mass of 20,600 and pI of 6. The optimum pH, optimum temperature, and pH stability of the chitinase were pH 6, 37.degree.C, and pH 5-7, respectively. The unique characteristics of the purified chitinase include low molecular mass and acidic pI. In the investigation of the inhibitory activity, it was found that the growth of F. oxysporum was 100% inhibited after incubation for 1 day with sterilized W-118 chitinase solution (5.6 units/mL).The chitinase hydrolyzates of chitin with low degrees of polymerization (DP) from 1 to 6 were analyzed by HPLC. Longer reaction time led to the generation of chitin oligosaccharides with lower DP. The chitin oligosaccharides were examined for their inhibitory effects on Fusarium oxysporum and human leukemia cell lines.
Relation:
2007年 台灣幾丁質幾丁聚醣研討會論文集=Proceedings of 2007 Taiwan Chitin and Chitosan Symposium, 2p.
