Biodegradation of Poly(ß-hydroxybutyrate) by a novel isolate of Streptomyces bangladeshensis 77T-4

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题名:	Biodegradation of Poly(ß-hydroxybutyrate) by a novel isolate of Streptomyces bangladeshensis 77T-4
作者:	Ke-Jen Hsu;Min Tseng;Don, Trong-Ming;Mei-Kwei Yang
贡献者:	淡江大學化學工程與材料工程學系
关键词:	PHB-degraders;PHB depolymerases;Streptomyces bangladeshensis;Thermophilic actinomycetes
日期:	2012-07
上传时间:	2013-01-17 09:32:36 (UTC+8)
出版者:	Taipei: Academia Sinica * Institute of Plant and Microbial Biology
摘要:	A thermophilic Streptomyces bangladeshensis isolate designated 77T-4 that can degrade poly(β-hydroxybutyrate) (PHB) was isolated from a soil sample in Taiwan. Isolate 77T-4 grew well in ureafructose oatmeal medium and produced a clear zone around the colony when grown on an agar plate containing emulsified PHB, indicating the presence of an extracellular PHB depolymerases. A PHB-degrading enzyme was purified to homogeneity from the culture supernatant. The molecular weight of the PHB-degrading enzyme was estimated to be approximately 40 kDa. A small portion of the N-terminal sequence (Ala-Val-Pro-Leu-Thr-Arg ) of the purified enzyme was determined and found to share a significant homology with that of the PHB depolymerase of Streptomyces sp. MG.
關聯:	Botanical Studies 53(3), pp.307-313
显示于类别:	[化學工程與材料工程學系暨研究所] 期刊論文

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