Taipei: Academia Sinica * Institute of Plant and Microbial Biology
A thermophilic Streptomyces bangladeshensis isolate designated 77T-4 that can degrade
poly(β-hydroxybutyrate) (PHB) was isolated from a soil sample in Taiwan. Isolate 77T-4 grew well in ureafructose oatmeal medium and produced a clear zone around the colony when grown on an agar plate containing emulsified PHB, indicating the presence of an extracellular PHB depolymerases. A PHB-degrading enzyme was purified to homogeneity from the culture supernatant. The molecular weight of the PHB-degrading enzyme was estimated to be approximately 40 kDa. A small portion of the N-terminal sequence (Ala-Val-Pro-Leu-Thr-Arg ) of the purified enzyme was determined and found to share a significant homology with that of the PHB depolymerase of Streptomyces sp. MG.