English  |  正體中文  |  简体中文  |  全文筆數/總筆數 : 61875/94645 (65%)
造訪人次 : 1636571      線上人數 : 7
RC Version 7.0 © Powered By DSPACE, MIT. Enhanced by NTU Library & TKU Library IR team.
搜尋範圍 查詢小技巧:
  • 您可在西文檢索詞彙前後加上"雙引號",以獲取較精準的檢索結果
  • 若欲以作者姓名搜尋,建議至進階搜尋限定作者欄位,可獲得較完整資料
  • 進階搜尋
    請使用永久網址來引用或連結此文件: https://tkuir.lib.tku.edu.tw/dspace/handle/987654321/80051

    題名: Preparation of bi-continuous macroporous polyamide copolymer membranes for cell culture
    作者: Don, Trong-Ming;Yi-Chun Hsu;Hung-Yin Tai;Earl Fu;Liao-Ping Cheng
    貢獻者: 淡江大學化學工程與材料工程學系
    關鍵詞: Chitosan;Porous membrane;Nylon copolymer;Cell culture;Scaffold
    日期: 2012-10-01
    上傳時間: 2013-01-17 09:12:53 (UTC+8)
    出版者: Amsterdam: Elsevier BV
    摘要: Skinless bi-continuous macroporous membranes with extremely high porosity, ∼80%, were prepared by the non-solvent induced phase separation (NIPS) process, from casting dopes composed of Elvamide (co-polymer of Nylon-6, -66, and -610), chitosan, and formic acid that were immersed in a water bath maintained at 10 °C. The Chitosan, while functioned as a pore former, migrated along with out-diffusing formic acid into the bath during the NIPS process, thereby, breaking the top gel-layer and the boundaries between cellular pores to yield a morphology featuring interpenetration networks of pores and polymer host. The amount of chitosan added to the dope affected significantly the pore size and porosity of the membranes formed, as revealed by SEM observations. At the highest chitosan/Elvamide ratio of 0.155, very large pore size (∼30 μm) and porosity (83%) were achieved, whereas at the lowest ratio of 0.1, the membrane became asymmetric, and the pore size was reduced to ∼15 μm. 1H NMR analyses indicated that chitosan was completely removed during the precipitation process. Furthermore, L929 cells were cultured on various porous membranes. It is interesting to find that this cell was able to dwell on the pore walls in the cross sectional region, although with a smaller proliferation than on a flat nonporous surface.
    關聯: Journal of Membrane Science 415–416, pp.784–792
    DOI: 10.1016/j.memsci.2012.05.070
    顯示於類別:[化學工程與材料工程學系暨研究所] 期刊論文


    檔案 描述 大小格式瀏覽次數
    J Memb Sic CS- Evalar 2012 DOI.pdf2414KbAdobe PDF763檢視/開啟



    DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library & TKU Library IR teams. Copyright ©   - 回饋