Biodegradation of shellfish wastes and production of chitosanases by a squid pen-assimilating bacterium, Acinetobacter calcoaceticus TKU024

doi:10.1007/s10532-011-9453-5

淡江大學機構典藏 > 理學院 > 生命科學研究所 > 期刊論文 > Item 987654321/80002

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題名:	Biodegradation of shellfish wastes and production of chitosanases by a squid pen-assimilating bacterium, Acinetobacter calcoaceticus TKU024
作者:	Wang, San-Lang;Tseng, Wan-Nine;Liang, Tzu-Wen
貢獻者:	淡江大學生命科學研究所;淡江大學化學學系
關鍵詞:	Chitosanase;Squid pen wastes;Acinetobacter calcoaceticus;Reducing sugar
日期:	2011-09
上傳時間:	2013-01-16 14:37:47 (UTC+8)
出版者:	Dordrecht: Springer Netherlands
摘要:	Two chitosanases (CHSA1 and CHSA2) were purified from the culture supernatant of Acinetobacter calcoaceticus TKU024 with squid pen as the sole carbon/nitrogen source. The molecular masses of CHSA1 and CHSA2 determined by SDS-PAGE were approximately 27 and 66 kDa, respectively. The optimum pH, optimum temperature, pH stability, and thermal stability of CHSA1 and CHSA2 were (pH 6, 50°C, pH 4–10, ＜90°C) and (pH 7, 60°C, pH 6–11, ＜70°C), respectively. CHSA1 and CHSA2 had broad pH and thermal stability. CHSA1 and CHSA2 were both inhibited by EDTA and were inhibited completely by 5 mM Mn2+. CHSA1 and CHSA2 degraded chitosan with DD ranging from 60 to 98%, and also degraded some chitin. The most susceptible substrate was 60% deacetylated chitosan. Furthermore, TKU024 culture supernatant (1.5% SPP) incubated for 5 days has the most reducing sugars (0.63 mg/ml). With this method, we have shown that shellfish wastes may have a great potential for the production of bioactive materials.
關聯:	Biodegradation 22(5), pp.939-948
DOI:	10.1007/s10532-011-9453-5
顯示於類別:	[生命科學研究所] 期刊論文 [化學學系暨研究所] 期刊論文

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