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    Please use this identifier to cite or link to this item: https://tkuir.lib.tku.edu.tw/dspace/handle/987654321/80002

    Title: Biodegradation of shellfish wastes and production of chitosanases by a squid pen-assimilating bacterium, Acinetobacter calcoaceticus TKU024
    Authors: Wang, San-Lang;Tseng, Wan-Nine;Liang, Tzu-Wen
    Contributors: 淡江大學生命科學研究所;淡江大學化學學系
    Keywords: Chitosanase;Squid pen wastes;Acinetobacter calcoaceticus;Reducing sugar
    Date: 2011-09
    Issue Date: 2013-01-16 14:37:47 (UTC+8)
    Publisher: Dordrecht: Springer Netherlands
    Abstract: Two chitosanases (CHSA1 and CHSA2) were purified from the culture supernatant of Acinetobacter calcoaceticus TKU024 with squid pen as the sole carbon/nitrogen source. The molecular masses of CHSA1 and CHSA2 determined by SDS-PAGE were approximately 27 and 66 kDa, respectively. The optimum pH, optimum temperature, pH stability, and thermal stability of CHSA1 and CHSA2 were (pH 6, 50°C, pH 4–10, <90°C) and (pH 7, 60°C, pH 6–11, <70°C), respectively. CHSA1 and CHSA2 had broad pH and thermal stability. CHSA1 and CHSA2 were both inhibited by EDTA and were inhibited completely by 5 mM Mn2+. CHSA1 and CHSA2 degraded chitosan with DD ranging from 60 to 98%, and also degraded some chitin. The most susceptible substrate was 60% deacetylated chitosan. Furthermore, TKU024 culture supernatant (1.5% SPP) incubated for 5 days has the most reducing sugars (0.63 mg/ml). With this method, we have shown that shellfish wastes may have a great potential for the production of bioactive materials.
    Relation: Biodegradation 22(5), pp.939-948
    DOI: 10.1007/s10532-011-9453-5
    Appears in Collections:[Graduate Institue of Life Sciences] Journal Article
    [Graduate Institute & Department of Chemistry] Journal Article

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