Fermented and enzymatic production of chitin/chitosan oligosaccharides by extracellular chitinases from Bacillus cereus TKU027

doi:10.1016/j.carbpol.2012.06.077

淡江大學機構典藏 > 理學院 > 化學學系暨研究所 > 期刊論文 > Item 987654321/79672

jsp.display-item.identifier=請使用永久網址來引用或連結此文件: https://tkuir.lib.tku.edu.tw/dspace/handle/987654321/79672

题名:	Fermented and enzymatic production of chitin/chitosan oligosaccharides by extracellular chitinases from Bacillus cereus TKU027
作者:	Wang, San-Lang;Liu, Chin-Pei;Liang, Tzu-Wen
贡献者:	淡江大學生命科學研究所;淡江大學化學學系
关键词:	Chitinase;Chitin oligosaccharides;Ahrimp heads;Bacillus cereus;Fermented;Enzymatic hydrolysis
日期:	2012-10-15
上传时间:	2013-01-08 14:14:53 (UTC+8)
出版者:	Kidlington: Pergamon
摘要:	Two chitinases, Chi I and Chi II, were purified from the culture supernatant of Bacillus cereus TKU027 with shrimp head powder (SHP) as the sole carbon/nitrogen source. The molecular masses of Chi I and Chi II determined using SDS–PAGE were approximately 65 kDa and 63 kDa, respectively. Chi I toward various surfactants showed high stability, such as SDS, Tween 20, Tween 40 and Triton X-100, and these surfactants were stimulator of Chi I chitinase activity. Concomitant with the production of Chi I and Chi II, chitin oligosaccharides were also observed in the culture supernatant, including chitobiose, chitotriose, chitotetrose and chitopentose at concentrations of 0.44 mg/mL, 0.08 mg/mL, 0.09 mg/mL and 0.43 mg/mL, respectively. Chitosan with 60% deacetylation was degraded by TKU027 crude enzyme to prepare chitooligosaccharides. MALDI-TOF MS analysis of the enzymatic hydrolyzates indicated that the products were mainly chitooligosaccharides with degree of polymerization (DP) in the 4–9 range.
關聯:	Carbohydrate Polymers 90(3), pp.1305–1313
DOI:	10.1016/j.carbpol.2012.06.077
显示于类别:	[化學學系暨研究所] 期刊論文

文件中的档案:

档案	描述	大小	格式	浏览次数
2012CARPTKU027LiuCP.pdf		636Kb	Adobe PDF	821	检视/开启
index.html		0Kb	HTML	368	检视/开启

在機構典藏中所有的数据项都受到原著作权保护.

TAIR相关文章

数据加载中.....