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    Please use this identifier to cite or link to this item: https://tkuir.lib.tku.edu.tw/dspace/handle/987654321/79672

    Title: Fermented and enzymatic production of chitin/chitosan oligosaccharides by extracellular chitinases from Bacillus cereus TKU027
    Authors: Wang, San-Lang;Liu, Chin-Pei;Liang, Tzu-Wen
    Contributors: 淡江大學生命科學研究所;淡江大學化學學系
    Keywords: Chitinase;Chitin oligosaccharides;Ahrimp heads;Bacillus cereus;Fermented;Enzymatic hydrolysis
    Date: 2012-10-15
    Issue Date: 2013-01-08 14:14:53 (UTC+8)
    Publisher: Kidlington: Pergamon
    Abstract: Two chitinases, Chi I and Chi II, were purified from the culture supernatant of Bacillus cereus TKU027 with shrimp head powder (SHP) as the sole carbon/nitrogen source. The molecular masses of Chi I and Chi II determined using SDS–PAGE were approximately 65 kDa and 63 kDa, respectively. Chi I toward various surfactants showed high stability, such as SDS, Tween 20, Tween 40 and Triton X-100, and these surfactants were stimulator of Chi I chitinase activity. Concomitant with the production of Chi I and Chi II, chitin oligosaccharides were also observed in the culture supernatant, including chitobiose, chitotriose, chitotetrose and chitopentose at concentrations of 0.44 mg/mL, 0.08 mg/mL, 0.09 mg/mL and 0.43 mg/mL, respectively. Chitosan with 60% deacetylation was degraded by TKU027 crude enzyme to prepare chitooligosaccharides. MALDI-TOF MS analysis of the enzymatic hydrolyzates indicated that the products were mainly chitooligosaccharides with degree of polymerization (DP) in the 4–9 range.
    Relation: Carbohydrate Polymers 90(3), pp.1305–1313
    DOI: 10.1016/j.carbpol.2012.06.077
    Appears in Collections:[Graduate Institute & Department of Chemistry] Journal Article

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