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    Please use this identifier to cite or link to this item: http://tkuir.lib.tku.edu.tw:8080/dspace/handle/987654321/78267

    Title: Cloning and Expression of Bioactive Human Granulocyte Colony Stimulating Factor in Pichia pastoris
    Authors: 簡素芳;Chien, Su-Fang
    Contributors: 淡江大學化學學系
    Keywords: Pichia pastoris;Neutropenia;HL 60;rhG-CSF-Recombinant human granulocyte colony stimulating factor;hG-CSF-Human granulocyte colony stimulating factor
    Date: 2010-08-01
    Issue Date: 2013-07-11 11:26:34 (UTC+8)
    Publisher: 臺北市:中國化學學會
    Abstract: For over ten years, recombinant human Granulocyte Colony Stimulating Factor (hG-CSF) has been
    broadly used in clinical applications. Many sources of G-CSF have the disadvantage of being in
    intracellular or aggregated form. In this paper, we report the molecular cloning and gene expression in the
    protease resistant strain of yeast (SMD1168). More than 90% of the G-CSF protein is in secreted form and
    about 100 mg of the biologic active human G-CSF can be obtained from two liters of bioreactor. The recombinant G-CSF promotes HL-60 (Human promyelocytic cell) cell proliferation and elevates the mito-chondrial succinate dehydrogenase activity. The dose effects of rG-CSF on cell proliferation responses to the total numbers of the receptors on the cell surfaces
    have been investigated and the quantitative results were obtained. The receptors are saturated byG-CSF atthe50 ng/mLfor 1x
    10^5 cells. The prompt increasing in the succinate dehydrogenas activity was also observed. This effect is comparable to that from the commercial source. We have successfully cloned and expressed a bioactive rhG-CSF from the medium in
    the Pichia pastoris system. This specific product is expected to meet the clinical applications in the treatments of neutropenia, acute cerebral ischemia and other neuro-degenerated diseases.
    Relation: Journal of the Chinese Chemical Society=中國化學會會誌 57(4B), pp.850-856
    DOI: 10.1002/jccs.201000118
    Appears in Collections:[Graduate Institute & Department of Chemistry] Journal Article

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