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    题名: Rhodamine-ethylenediol, a novel vital fluorescent probe for labeling alkaline phosphatase-rich organelles
    作者: Chen, Yau-hung;Chiou, Chien-hui;Chen, Wei-li;Jhou, Yu-ru;Lee, Ya-ting;Cheng, Chien-chung
    贡献者: 淡江大學化學學系;淡江大學企業管理學系
    关键词: Rhodamine;Zebrafish;Fluorescence;Neuromast;Villus;Alkaline phophatase
    日期: 2010-12-01
    上传时间: 2013-07-29 13:42:37 (UTC+8)
    出版者: 臺北市:中國化學學會
    摘要: Zebrafish have received considerable attention as an organism-based model in the development of pharmacological agents. Many small moleculesapplied to zebrafish show important behaviours and may constitute new kinds of markers for clinical purposes. Analysis of these molecules can facilitate the development of useful tools for monitoring environmental changes. Many chemicals that are toxic to the environment are known to influence the sensory systems of humans and fish. One important sensory system in all fish is the lateral line organ, which is readily accessible for the assessment of environmental changes. Neuromasts, which are located on the surface of the fish body, are one of the major components of the lateral lines of the zebrafish. Copper enriched water is known to affect the olfactory system in fish. Therefore, small molecules that induce specific patterns in the neuromasts of zebrafish should provide an important animal model with which to explore the effects of environmental changes on the sensory system.Recently, chemical sensors based on the rhodamine skeleton have been designed to specifically detect metal ions, such as Cu(II) and Fe(III)/Hg(II), in zebrafish. However, there has been no report of these rhodamine derivatives used in the specific recognition of the sensory system of zebrafish. Commonly, the sensory system is studied with antibody staining assays of scarified fish. Here, we report that a new rhodamine derivative can be used as a fluorescent chemical probe to visualize the neuromasts and intestinal villi of living zebrafish. Based on the specific recognition of this area in zebrafish, we narrowed the possible enzymes targeted by this rhodamine probe to alkaline phosphatase and confirmed this with a binding assay. It is a well-recognized challenge to develop a fluorescent chemical probe that specifically recognizes a particular enzyme. Furthermore, the transfer of phosphate groups to certain enzymes can activate their catalytic reactivity, triggering a cascade reaction in a signal transduction pathway. The alkaline phosphatase specific recognition by this rhodamine derivative may be applicable to clinical purposes.
    關聯: Journal of the Chinese Chemical Society=中國化學會會誌 57(6), pp.1257-1261
    DOI: 10.1002/jccs.201000186
    显示于类别:[企業管理學系暨研究所] 期刊論文
    [化學學系暨研究所] 期刊論文

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