Inductive properties of polypyridyl ruthenium complexes significantly regulate various protein distributions in Escherichia coli

doi:10.1016/j.jinorgbio.2011.03.017

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Title:	Inductive properties of polypyridyl ruthenium complexes significantly regulate various protein distributions in Escherichia coli
Authors:	Ho, Ming-yi;Chiou, Min-lun;Du, Wan-shan;Chang, Fang-ying;Chen, Yau-hung;Weng, Yu-jong;Cheng, Chien-chung
Contributors:	淡江大學化學學系
Keywords:	Ruthenium;2D gel;Polypyridyl;Metalloproteomic;LC/MS;Ru-protein interaction
Date:	2011
Issue Date:	2012-06-14 09:06:50 (UTC+8)
Publisher:	Philadelphia: Elsevier Inc.
Abstract:	Ruthenium complexes with similar octahedral structures but different intrinsic inductive properties significantly influence the total cellular protein distributions, which may affect different metabolic pathways. A systematic study of the relationship between ruthenium complexes and Escherichia coli was undertaken, using two-dimensional gel electrophoresis analysis and the identification of various proteins by mass data mining. Based on the low similarities (<40%) between the total protein distributions, the inductive properties of the ruthenium complexes are relevant to the formation of the protein-Ru interaction in addition to the Ru-DNA interaction. Two major protein functions in E. coli BL21 that were reduced by compound 1 were oxidoreductases and transporters, corresponding to 29% and 25% of the 24 down-regulated proteins. The main biological processes of the proteins down-regulated by compound 1 were related to carbohydrate reactions, including in transport, tricarboxylic acid (TCA) cycle, glycolysis, and gluconeogenesis. All four ruthenium complexes shared similar up-regulated proteins, including clpB and kpyk1, and down-regulated similar proteins, including ompA and ybbN. This result supports that the presence of Ru-protein interactions is a major factor affecting bacteria growth, and particularly transport and carbohydrate-related reactions.
Relation:	Journal of Inorganic Biochemistry 105(6), pp.902-910
DOI:	10.1016/j.jinorgbio.2011.03.017
Appears in Collections:	[Graduate Institute & Department of Chemistry] Journal Article

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