Surface modification of microporous PVDF membranes for neuron culture

doi:10.1016/j.memsci.2009.12.009

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Title:	Surface modification of microporous PVDF membranes for neuron culture
Authors:	Young, Tai-Horng;Chang, Hsu-Hsien;Lin, Dar-Jong;Cheng, Liao-Ping
Contributors:	淡江大學化學工程與材料工程學系
Keywords:	Poly(vinylidene fluoride);Porous membrane;Immobilization;l-lysine;Neuron culture
Date:	2010-03
Issue Date:	2012-06-14 09:03:26 (UTC+8)
Publisher:	Amsterdam: Elsevier BV
Abstract:	Microporous poly(vinylidene fluoride) (PVDF) membranes with either dense or porous surface were prepared by isothermal immersion–precipitation of a casting solution in coagulation baths of different strengths. Onto the membranesurface, amino acid (l-lysine) or 1,6-hexanediamine (HMDA) was chemically immobilized. The membrane was first grafted with poly(glycidyl methacrylate) (PGMA) by means of plasma-induced free radical polymerization. Then, l-lysine or 1,6-hexanediamine was reacted with epoxy groups in the grafted PGMA to create an ECM environment suited to cell culture. Neuronal cells were cultivated on the formed pristine and surface-modified PVDFmembranes. It is found that neurons tend to aggregate into large clusters with neuritic branches on pristine PVDFmembranes, whereas for those cultured on the PGMA/PVDFmembranes, serious aggregation takes place as well, but without neurites being formed. In contrast, neurons did not aggregate on the l-lysine or HMDA-immobilized membrane. It is also interesting to find that a neuritic network inter-connecting cells is constructed for the former, yet no neurite is observed for the latter membrane. Such phenomenon is thought to be associated with the extra –COOH group in l-lysine with respect to HMDA.
Relation:	Journal of Membrane Science 350(1-2), pp.32-41
DOI:	10.1016/j.memsci.2009.12.009
Appears in Collections:	[Graduate Institute & Department of Chemical and Materials Engineering] Journal Article

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