English  |  正體中文  |  简体中文  |  Items with full text/Total items : 49279/83828 (59%)
Visitors : 7145956      Online Users : 74
RC Version 7.0 © Powered By DSPACE, MIT. Enhanced by NTU Library & TKU Library IR team.
Scope Tips:
  • please add "double quotation mark" for query phrases to get precise results
  • please goto advance search for comprehansive author search
  • Adv. Search
    HomeLoginUploadHelpAboutAdminister Goto mobile version
    Please use this identifier to cite or link to this item: http://tkuir.lib.tku.edu.tw:8080/dspace/handle/987654321/77223


    Title: Surface modification of microporous PVDF membranes for neuron culture
    Authors: Young, Tai-Horng;Chang, Hsu-Hsien;Lin, Dar-Jong;Cheng, Liao-Ping
    Contributors: 淡江大學化學工程與材料工程學系
    Keywords: Poly(vinylidene fluoride);Porous membrane;Immobilization;l-lysine;Neuron culture
    Date: 2010-03
    Issue Date: 2012-06-14 09:03:26 (UTC+8)
    Publisher: Amsterdam: Elsevier BV
    Abstract: Microporous poly(vinylidene fluoride) (PVDF) membranes with either dense or porous surface were prepared by isothermal immersion–precipitation of a casting solution in coagulation baths of different strengths. Onto the membranesurface, amino acid (l-lysine) or 1,6-hexanediamine (HMDA) was chemically immobilized. The membrane was first grafted with poly(glycidyl methacrylate) (PGMA) by means of plasma-induced free radical polymerization. Then, l-lysine or 1,6-hexanediamine was reacted with epoxy groups in the grafted PGMA to create an ECM environment suited to cell culture. Neuronal cells were cultivated on the formed pristine and surface-modified PVDFmembranes. It is found that neurons tend to aggregate into large clusters with neuritic branches on pristine PVDFmembranes, whereas for those cultured on the PGMA/PVDFmembranes, serious aggregation takes place as well, but without neurites being formed. In contrast, neurons did not aggregate on the l-lysine or HMDA-immobilized membrane. It is also interesting to find that a neuritic network inter-connecting cells is constructed for the former, yet no neurite is observed for the latter membrane. Such phenomenon is thought to be associated with the extra –COOH group in l-lysine with respect to HMDA.
    Relation: Journal of Membrane Science 350(1-2), pp.32-41
    DOI: 10.1016/j.memsci.2009.12.009
    Appears in Collections:[化學工程與材料工程學系暨研究所] 期刊論文

    Files in This Item:

    File Description SizeFormat
    0376-7388_350(1-2)p32-41.pdf2697KbAdobe PDF169View/Open
    0376-7388_350(1-2)p32-41.pdf2697KbAdobe PDF0View/Open

    All items in 機構典藏 are protected by copyright, with all rights reserved.


    DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library & TKU Library IR teams. Copyright ©   - Feedback