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    Title: Embryonic exposure to diclofenac disturbs actin organization and leads to myofibril misalignment
    Authors: Chen, Ying-hsin;Chang, Chao-yuan;Wang, Yun-hsin;Wen, Chi-chung;Chen, Yun-chen;Hu, Sheng-chuan;Yu, Dah-shyong;Chen, Yau-hung
    Contributors: 淡江大學生命科學研究所;淡江大學數學學系
    Keywords: actin;diclofenac;myotoxic;myod;zebrafish
    Date: 2011-04
    Issue Date: 2012-05-24 11:17:34 (UTC+8)
    Publisher: Hoboken: John Wiley & Sons, Inc.
    Abstract: The objective of this study was to investigate the embryotoxicity of diclofenac. Zebrafish (Danio rerio) embryos at 12 hpf were treated with different dosages of diclofenac (0–2,000 ppm) for different time courses (12–72 hr). Results showed no evident differences in survival rates or morphological changes between the mock-treated control (0 ppm) zebrafish embryos and those with 1-ppm diclofenac-exposure (12–24, 12–36 hpf). In contrast, after higher doses (5 and 10 ppm) of exposure, embryos displayed some defective phenotypes, including malformed somite boundary, a twisted body axis, and shorter body length. In addition, diclofenac-treated embryos exhibited significantly reduced frequencies of spontaneous in-chorion contractions in comparison with mock-control littermates (mock-control: 13.20±2.24 vs. 5–10 ppm diclofenac: 6.66±1.35–3.03±1.84). Subtle changes were easily observed by staining with specific monoclonal antibodies F59 and phalloidin to detect morphological changes in muscle fibers and formation of F-actin, respectively. Our data show that diclofenac treatment disturbs actin organization and muscle fiber alignment, thus causing malformed somite phenotypes.
    Relation: Birth Defects Research Part B: Developmental and Reproductive Toxicology 92(2), pp.139-147
    DOI: 10.1002/bdrb.20292
    Appears in Collections:[化學學系暨研究所] 期刊論文
    [生命科學研究所] 期刊論文
    [數學學系暨研究所] 期刊論文

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