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    题名: UV-induced fin damage in zebrafish as a system for evaluating the chemopreventive potential of broccoli and cauliflower extracts
    作者: Chen, Yau-hung;Wen, Chi-chung;Lin, Chun-yun;Chou, Chi-yuan;Yang, Zhi-shiang;Wang, Yun-hsin
    贡献者: 淡江大學生命科學研究所;淡江大學數學學系
    关键词: Broccoli;cauliflower;fin;skin cancer;UVB;zebra fish
    日期: 2011-01
    上传时间: 2012-05-24 11:13:43 (UTC+8)
    出版者: New York: Informa Healthcare
    摘要: This study applied broccoli and cauliflower extracts (whole, floret, and stem) to zebrafish larvae in parallel to receive 100 mJ/cm2 of UVB six times, and recorded their fin malformation phenotypes. Chemopreventive effects of each group, including UVB, whole-, floret-, and stem-extracts of broccoli and cauliflower on fin development were evaluated using Kaplan-Meier analysis, log-rank test, and Cox proportional hazards regression. Results showed that (1) zebrafish fins in the UVB + whole broccoli extract group are 6.20~9.32-times more likely to return to normal fins than ones in the UVB only group, but fins in the UVB + whole cauliflower extract group are only 5.13~11.10-times more likely to recover, indicated that whole broccoli and cauliflower extract had similar chemopreventive ability on fin development; and (2) the broccoli stem has the highest antioxidant capacity among other groups. In conclusion, zebrafish can be used as a system for evaluating the efficacy of other UVB protective compounds.
    關聯: Toxicology Mechanisms and Methods 21(1), pp.63-69
    DOI: 10.3109/15376516.2010.529191
    显示于类别:[生命科學研究所] 期刊論文

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