Taro α-galactosidase: A new gene product for blood conversion

淡江大學機構典藏 > 理學院 > 生命科學研究所 > 期刊論文 > Item 987654321/76985

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题名:	Taro α-galactosidase: A new gene product for blood conversion
作者:	Chern, Ming-kai;Li, Huang-yi;Chen, Po-fan;Chien, Su-fang
贡献者:	淡江大學化學學系;淡江大學生命科學研究所
关键词:	Taro α-galactosidase;Colocasia esculenta;Pichia pastoris expression;Mass spectrometry;Red blood cell
日期:	2012-04
上传时间:	2012-05-23 14:30:09 (UTC+8)
出版者:	Amsterdam: Elsevier BV
摘要:	The N-terminal sequence and internal sequences of the purified taro α-galactosidase (α-Gal) were determined using tandem mass spectrometry. By using reverse transcriptase PCR (RT-PCR), 5′ and 3′ rapid amplification of cDNA ends (RACE) with designed, degenerate primers, a novel cDNA sequence was obtained. The recombinant taro α-Gal not only hydrolyzes α1→4 linked galactosyl residues, which are accumulated in the tissues from patients with Fabry disease, but also hydrolyzes the α1→3 linked galactoside of B red blood cells (RBC). The recombinant taro α-Gal provides an ideal enzyme source for biomedical systems.
關聯:	Biocatalysis and Agricultural Biotechnology 1(2),pp.135-139
DOI:	10.1016/j.bcab.2011.09.004
显示于类别:	[生命科學研究所] 期刊論文

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