Taro α-galactosidase: A new gene product for blood conversion

機構典藏 > College of Sciences > Graduate Institue of Life Sciences > Journal Article > Item 987654321/76985

Please use this identifier to cite or link to this item: https://tkuir.lib.tku.edu.tw/dspace/handle/987654321/76985

Title:	Taro α-galactosidase: A new gene product for blood conversion
Authors:	Chern, Ming-kai;Li, Huang-yi;Chen, Po-fan;Chien, Su-fang
Contributors:	淡江大學化學學系;淡江大學生命科學研究所
Keywords:	Taro α-galactosidase;Colocasia esculenta;Pichia pastoris expression;Mass spectrometry;Red blood cell
Date:	2012-04
Issue Date:	2012-05-23 14:30:09 (UTC+8)
Publisher:	Amsterdam: Elsevier BV
Abstract:	The N-terminal sequence and internal sequences of the purified taro α-galactosidase (α-Gal) were determined using tandem mass spectrometry. By using reverse transcriptase PCR (RT-PCR), 5′ and 3′ rapid amplification of cDNA ends (RACE) with designed, degenerate primers, a novel cDNA sequence was obtained. The recombinant taro α-Gal not only hydrolyzes α1→4 linked galactosyl residues, which are accumulated in the tissues from patients with Fabry disease, but also hydrolyzes the α1→3 linked galactoside of B red blood cells (RBC). The recombinant taro α-Gal provides an ideal enzyme source for biomedical systems.
Relation:	Biocatalysis and Agricultural Biotechnology 1(2),pp.135-139
DOI:	10.1016/j.bcab.2011.09.004
Appears in Collections:	[Graduate Institue of Life Sciences] Journal Article

Files in This Item:

File	Description	Size	Format
index.html		0Kb	HTML	566	View/Open
Taro α-galactosidase; A new gene product for blood conversion, Biocatalysis and Agricultural Biotechnology_BAB(1)135_1-s2.0-S1878818112000229-main.pdf	論文電子	794Kb	Adobe PDF	1497	View/Open