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    题名: Taro α-galactosidase: A new gene product for blood conversion
    作者: Chern, Ming-kai;Li, Huang-yi;Chen, Po-fan;Chien, Su-fang
    贡献者: 淡江大學化學學系;淡江大學生命科學研究所
    关键词: Taro α-galactosidase;Colocasia esculenta;Pichia pastoris expression;Mass spectrometry;Red blood cell
    日期: 2012-04
    上传时间: 2012-05-23 14:30:09 (UTC+8)
    出版者: Amsterdam: Elsevier BV
    摘要: The N-terminal sequence and internal sequences of the purified taro α-galactosidase (α-Gal) were determined using tandem mass spectrometry. By using reverse transcriptase PCR (RT-PCR), 5′ and 3′ rapid amplification of cDNA ends (RACE) with designed, degenerate primers, a novel cDNA sequence was obtained. The recombinant taro α-Gal not only hydrolyzes α1→4 linked galactosyl residues, which are accumulated in the tissues from patients with Fabry disease, but also hydrolyzes the α1→3 linked galactoside of B red blood cells (RBC). The recombinant taro α-Gal provides an ideal enzyme source for biomedical systems.
    關聯: Biocatalysis and Agricultural Biotechnology 1(2),pp.135-139
    DOI: 10.1016/j.bcab.2011.09.004
    显示于类别:[生命科學研究所] 期刊論文


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