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    題名: Resveratrol Treatment Attenuates the Wound-Induced Inflammation in Zebrafish Larvae through the Suppression of Myeloperoxidase Expression
    作者: Liao, Yun-Feng;Chiou, Min-Chuan;Tsai, Jen-Ning;Wen, Chi-Chung;Wang, Yun-Hsin;Cheng, Chien-Chung;Chen, Yau-Hung
    貢獻者: 淡江大學數學學系;淡江大學化學學系
    關鍵詞: cyclooxygenase;inflammation;myeloperoxidase;resveratrol;zebrafish
    日期: 2011-07
    上傳時間: 2012-05-23 12:40:03 (UTC+8)
    出版者: 臺北市:行政院衛生署藥物食品檢驗局
    摘要: Resveratrol, a polyphenolic phytoalexin found in many plants, was reported to exhibit anti-inflammatory effects, but its molecular mechanism is not fully understood. This study was aimed to investigate the anti-inflammatory effects of resveratrol in vivo using a zebrafish model of wound-induced inflammation. Caudal fin-wounded zebrafish larvae were treated with resveratrol for 8 h. Neutrophil recruitment was visualized in transgenic line “Tg (mpx:GFP)” expressing GFP-tagged neutrophil-specific myeloperoxidase (mpx). The enzymatic activity of Mpx was evaluated by histochemical staining. Relative mRNA levels of mpx and cyclooxegenase-2 (cox2) were quantified by qRT-PCR, and the protein expression levels of Mpx and Cox2 were detected by immunostaining. Results showed that wound-induced neutrophil recruitment in zebrafish was not affected by resveratrol, but Mpx enzymatic activity in zebrafish was significantly suppressed by resveratrol in a dose-dependent manner. Moreover, both mRNA and protein expression levels of Mpx and Cox2 were significantly down-regulated by resveratrol. Taken together, our results provide in vivo evidence that the anti-inflammatory effects of resveratrol on wound-induced inflammation are significantly mediated through the suppression of Mpx and Cox2 at both transcriptional and protein levels, rather than the down-regulation of neutrophil recruitment. In conclusion, this in vivo zebrafish model can be readily applied to screen other potential anti-inflammatory compounds at a whole-organism level.
    關聯: Journal of Food and Drug Analysis=藥物食品分析 19(2), pp.167-173
    顯示於類別:[化學學系暨研究所] 期刊論文

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