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    題名: Molecular structure and developmental expression of zebrafish atp2a genes
    作者: Lai, Yen-Yu;Pai, Chiung-Wen;Tsai, I-Ting;Chou, Chi-Yuan;Tsai, Chia-Ti;Chen, Yau-Hung
    貢獻者: 淡江大學化學學系
    關鍵詞: Calcium;Cardiogenesis;Muscle;SERCA;Zebrafish
    日期: 2011
    上傳時間: 2012-05-23 12:14:02 (UTC+8)
    出版者: Seoul: Genetics Society of Korea
    摘要: We isolated two atp2a genes, atp2a1 and atp2a2a, from embryonic zebrafish. Amino acid sequences deduced from zebrafish atp2a genes are aligned with orthologue proteins from other species, the results showed that they share high percentage of identities (82%–94%) and acidic pIs (5.03–5.33). Whole mount in situ hybridization experiments showed that atp2a1 and atp2a2a are maternal inherited genes which can be detected at 1-cell stage embryos and express in the entire animal pole from 6 hours post-fertilization (hpf) to 12 hpf. At the later stages (48–96 hpf), expression of atp2a1 was restricted in head and trunk muscles as well as in some neurons. In contrast to the strongly expression of atp2a1 in head muscle, expression of atp2a2a was detected in head muscle in a fainter manner. In addition, transcripts of atp2a2a were observed in the developing heart during early cardiogenesis. The present studies not only help us to comparatively analyze atp2a genes across species, but also provide useful information about expressions during early embryogenesis that will help in further investigations of functional studies of Atp2a in the future.
    關聯: Genes & Genomics 33(5), pp.541-548
    DOI: 10.1007/s13258-011-0013-5
    顯示於類別:[化學學系暨研究所] 期刊論文

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