This research project focuses on the formation and surface modification of poly(vinylidene fluoride) (PVDF) and nylon hollow fiber membranes, and their applications in the biomedical area. First of all, a series of hollow fiber membranes with different porous structures will be prepared by both isothermal wet-spining and dry-jet wet-spinning methods. The preparation parameters that affect the porous structures of the membranes (e.g., concentrations of the casting dope and the precipitation bath, additives, etc) will be studied. SEM, DSC and XRD measurement will be employed to characterize the formed hollow fiber membranes. Then, PVDF membranes will be grafted, on their surfaces, with poly(glycidyl methacrylate) (PGMA) using atom transfer radical polymerization (ATRP) method. Subsequently, DNA will be immobilized on the membranes by reaction with epoxy groups of the grafted PGMA. Various reaction parameters (e.g., reaction temperature, reactant concentrations, pH, etc.) will be the subjects of investigation. On the other hand, Nylon membranes will be surfaced modified by reaction with hexamethylene diisocyanate, HMDI) with the aids of stannum (Sn, catalyst). The remaining -NCO group of HMDI will be used to immobilized DNA on the membrane. It is hoped that this research will produce biomaterials that is of clinical significance.