English  |  正體中文  |  简体中文  |  Items with full text/Total items : 62379/95055 (66%)
Visitors : 2298059      Online Users : 168
RC Version 7.0 © Powered By DSPACE, MIT. Enhanced by NTU Library & TKU Library IR team.
Scope Tips:
  • please add "double quotation mark" for query phrases to get precise results
  • please goto advance search for comprehansive author search
  • Adv. Search
    HomeLoginUploadHelpAboutAdminister Goto mobile version
    Please use this identifier to cite or link to this item: https://tkuir.lib.tku.edu.tw/dspace/handle/987654321/74665

    Title: 防止HepG2肝癌細胞貼附之力學探討
    Other Titles: A mechanical study on the anti-attachment of HepG2 tumor cells
    Authors: 徐智文;Hsu, Chih-Wen
    Contributors: 淡江大學機械與機電工程學系碩士班
    楊龍杰;Yang, Lung-Jieh
    Keywords: 轉移;細胞貼附;肝門靜脈;Metastasis;ECIS;cell adhesion;portal vein
    Date: 2011
    Issue Date: 2011-12-28 19:14:28 (UTC+8)
    Abstract: 癌細胞透過入侵循環系統而達到轉移之過程,與細胞的脫離以及貼附機制有關,為了探討此一現象,本研究分為靜態實驗與動態實驗兩部分。靜態實驗利用電子細胞基質阻抗判斷技術(Electric Cell-substrate Impedance Sensing, ECIS)製作阻抗感測晶片,使用戊二醛交聯之明膠微圖案,吸引細胞貼附於電極上方,並監測HepG2細胞之形態與貼附變化。而藉由每隔1小時所得到的細胞貼附面積與阻抗變化之關係,可成為動態實驗參考之依據。
    For metastasis of tumor cell invade into the circulatory system, it have to do with tumor-cell of detachment and attachment. This work presents the first part of a new framework for preventing the tumor-cell of carcinoma in situ transition from one organ to others. Using an ECIS (electric cell-substrate impedance sensing) chip coated with glutaraldehyde (GA)-crosslinked gelatin patterns suitable for cell attachment, the author monitor the cell adhesion situation not only by optical microscope but also by electrical means. This cell-culture experiment with 1-hour time resolution so far demonstrates that the attachment moment for HepG2 on gelatin surface is no longer than 4 hours after the cell dosing and these tumor cells cannot stay on GA-crosslinked gelatin surface for more than 7 hours.
    The second part of experiment is dynamic. The author design an experiment and a microfluidic chip for investigating the relationship between the metastasis and the surface morphology of blood vessels. Using RIE (reactive ion etch) to change the inner wall morphology of PDMS. To different inner wall of PDMS roughness, the ability of cell adhesion is different. When tumor-cell through the microchannel, it may pass or get stuck. It’s mean that capillary may plugged by tumor-cell, or still smooth. Finally, to explore the adhesion of tumor-cell and blocking when tumor-cell into the liver through the hepatic portal vein. It can assist in the prevention and treatment of cancer, such as diet, drug, gene therapy research.
    Appears in Collections:[Graduate Institute & Department of Mechanical and Electro-Mechanical Engineering] Thesis

    Files in This Item:

    File SizeFormat

    All items in 機構典藏 are protected by copyright, with all rights reserved.

    DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library & TKU Library IR teams. Copyright ©   - Feedback