Immobilization of l-lysine on dense and porous poly(vinylidene fluoride) surfaces for neurons culture

doi:10.1016/j.desal.2007.09.079

機構典藏 > College of Engineering > Graduate Institute & Department of Chemical and Materials Engineering > Journal Article > Item 987654321/71833

Please use this identifier to cite or link to this item: https://tkuir.lib.tku.edu.tw/dspace/handle/987654321/71833

Title:	Immobilization of l-lysine on dense and porous poly(vinylidene fluoride) surfaces for neurons culture
Authors:	Lin, Dar-jong;Chang, Chi-lin;Chang, Hsu-hsien;Cheng, Liao-ping;Young, Tai-horng;Lu, Jui-nan
Contributors:	淡江大學化學工程與材料工程學系
Keywords:	Poly(vinylidene fluoride);Porous;Membrane;Immobilization;l-lysine
Date:	2008-03-01
Issue Date:	2011-10-24 01:47:05 (UTC+8)
Abstract:	Microporous poly(vinylidene fluoride) (PVDF) membranes with either dense or porous surface were prepared by isothermal immersion-precipitation of a casting solution in coagulation baths of different strengths. Onto the membrane surface, an amino acid (l-lysine) was immobilized by a dual-step chemical process. First, the membrane was grafted with poly(acrylic acid) (PAA) by means of plasma-induced free radical polymerization. Then, l-lysine was covalently bonded to the as-grafted PAA chains with the aid of a water-soluble carbodiimide, 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide hydrochloride (EDC). The highest attainable graft yield of PAA on PVDF membrane reached up to 0.64 mg/cm2. For immobilization of l-lysine on the membrane, the yields were found to depend on factors, such as concentration of EDC, activation time, and pH value. The maximal attainable immobilization yield was 0.65 μg/cm2. Furthermore, pheochromocytoma (PC12) cells were cultured on l-lysine/PAA/PVDF membranes. It was found that both the amount of l-lysine on the membrane and the surface structure had a marked influence on the cell activity. Thus, the present results could be useful for the development of strategies to promote the re-growth and regeneration of tissue in the nervous system.
Relation:	Desalination 234, pp.134-143
DOI:	10.1016/j.desal.2007.09.079
Appears in Collections:	[Graduate Institute & Department of Chemical and Materials Engineering] Journal Article

Files in This Item:

File	Description	Size	Format
Immobilization of l-lysine on dense and porous poly(vinylidene fluoride) surfaces for neurons culture.pdf		840Kb	Adobe PDF	23	View/Open

Loading...