Microbial reclamation of shellfish wastes for the production of chitinases

doi:10.1016/S0141-0229(00)00325-2

機構典藏 > College of Sciences > Graduate Institue of Life Sciences > Journal Article > Item 987654321/61862

Please use this identifier to cite or link to this item: https://tkuir.lib.tku.edu.tw/dspace/handle/987654321/61862

Title:	Microbial reclamation of shellfish wastes for the production of chitinases
Authors:	Wang, San-lang;Hwang, Jau-ren
Contributors:	淡江大學生命科學研究所
Keywords:	Shrimp shell;Chitin;Chitinase;Bacillus cereus;Bacillus alvei;Bacillus sphaericus
Date:	2001-03
Issue Date:	2013-02-27 09:38:47 (UTC+8)
Publisher:	PA: Elsevier Inc.
Abstract:	Shrimp and crab shell powder (SCSP), prepared by treating shellfish processing waste with boiling and crushing, was used as a substrate for isolating chitinolytic microorganisms. Three potential strains (E1, J1, and J1-1) were isolated and identified as Bacillus cereus, B. alvei, and B. sphaericus, respectively. Three extracellular chitinases (FB1, FB2, and FB3) were purified from the culture supernatants of Bacillus cereus E1, B. alvei J1, and B. sphaericus J1-1, respectively. The molecular weights of FB1, FB2, and FB3 were 71,000, 71,000, and 65,000, respectively, by SDS-PAGE. The pIs for FB1, FB2, and FB3 were 7.1, 7.2, and 7.4, respectively. The optimum pH, optimum temperature, pH stability, and thermal stability of FB1 were pH 9, 50°C, pH 7 to 10, and 70°C; those of FB2 were pH 9, 60°C, pH 5 to 9, and 70°C; and those of FB3 were pH 7, 50°C, pH 5 to 9, and 60°C. The activities of all enzymes were strongly inhibited by Hg2+ and completely inhibited by glutathione, dithiothreitol, and 2-mercaptoethanol.
Relation:	Enzyme and Microbial Technology 28(4-5), pp.376-382
DOI:	10.1016/S0141-0229(00)00325-2
Appears in Collections:	[Graduate Institue of Life Sciences] Journal Article

Files in This Item:

File	Size	Format
index.html	0Kb	HTML	60	View/Open

Loading...