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    題名: Microbial reclamation of shellfish wastes for the production of chitinases
    作者: Wang, San-lang;Hwang, Jau-ren
    貢獻者: 淡江大學生命科學研究所
    關鍵詞: Shrimp shell;Chitin;Chitinase;Bacillus cereus;Bacillus alvei;Bacillus sphaericus
    日期: 2001-03
    上傳時間: 2013-02-27 09:38:47 (UTC+8)
    出版者: PA: Elsevier Inc.
    摘要: Shrimp and crab shell powder (SCSP), prepared by treating shellfish processing waste with boiling and crushing, was used as a substrate for isolating chitinolytic microorganisms. Three potential strains (E1, J1, and J1-1) were isolated and identified as Bacillus cereus, B. alvei, and B. sphaericus, respectively. Three extracellular chitinases (FB1, FB2, and FB3) were purified from the culture supernatants of Bacillus cereus E1, B. alvei J1, and B. sphaericus J1-1, respectively. The molecular weights of FB1, FB2, and FB3 were 71,000, 71,000, and 65,000, respectively, by SDS-PAGE. The pIs for FB1, FB2, and FB3 were 7.1, 7.2, and 7.4, respectively. The optimum pH, optimum temperature, pH stability, and thermal stability of FB1 were pH 9, 50°C, pH 7 to 10, and 70°C; those of FB2 were pH 9, 60°C, pH 5 to 9, and 70°C; and those of FB3 were pH 7, 50°C, pH 5 to 9, and 60°C. The activities of all enzymes were strongly inhibited by Hg2+ and completely inhibited by glutathione, dithiothreitol, and 2-mercaptoethanol.
    關聯: Enzyme and Microbial Technology 28(4-5), pp.376-382
    DOI: 10.1016/S0141-0229(00)00325-2
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