淡江大學機構典藏:Item 987654321/61854
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    题名: Functional analysis of the proximal promoter regions of fish rhodopsin and myf-5 genes using transgenesis
    作者: 陳曜鴻;Chen, Yau-hung
    贡献者: 淡江大學生命科學研究所
    日期: 2002
    上传时间: 2011-10-17 00:56:56 (UTC+8)
    摘要: Little is known about the cis-acting elements controlling transcriptional activities of fish rhodopsin and myf-5 genes. Transgenic medaka and zebrafish were used to characterize promoters of carp (Cyprinus carpio) rhodopsin gene (cRh) and zebrafish (Danio rerio) myf-5 gene (myf-5), respectively. Transgenic medaka carrying different lengths of cRh upstream fragments fused with enhanced green fluorescence protein (EGFP) gene revealed several functional regions. Both upstream regions from ?1261 to ?163 bp (?1261/?163) and ?163/?138 contributed to enhance the retina-specific activities of cRh. The cNRE (?75/?63) and CSE (?52/?46) motifs located at the cRh proximal promoter were sufficient to drive the transgene expressed in retinae. The ?73/?68 within cNRE was also proved to be essential for the integrity of the minimal cis-regulatory elements. Regarding the myf-5, transgene expression in zebrafish embryos injected with different deletion fragments of zebrafish myf-5 upstream sequences showed that the ?2776/?2456 and ?1046/?844 regions contributed to the enhancer function. The ?290/?154 region might be involved in controlling the translocation of progenitor muscle cells. Moreover, the upstream ?82/?1 region was identified as a minimal promoter required for somite-specific expression. The ?82/?62 region was the most critical sequence for myf-5 specificity.
    關聯: Marine Technology 4, pp.247-255
    DOI: 10.1007/s10126-002-0016-y
    显示于类别:[化學學系暨研究所] 期刊論文

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