English  |  正體中文  |  简体中文  |  全文筆數/總筆數 : 56826/90592 (63%)
造訪人次 : 12130624      線上人數 : 169
RC Version 7.0 © Powered By DSPACE, MIT. Enhanced by NTU Library & TKU Library IR team.
搜尋範圍 查詢小技巧:
  • 您可在西文檢索詞彙前後加上"雙引號",以獲取較精準的檢索結果
  • 若欲以作者姓名搜尋,建議至進階搜尋限定作者欄位,可獲得較完整資料
  • 進階搜尋
    請使用永久網址來引用或連結此文件: http://tkuir.lib.tku.edu.tw:8080/dspace/handle/987654321/61854


    題名: Functional analysis of the proximal promoter regions of fish rhodopsin and myf-5 genes using transgenesis
    作者: 陳曜鴻;Chen, Yau-hung
    貢獻者: 淡江大學生命科學研究所
    日期: 2002
    上傳時間: 2011-10-17 00:56:56 (UTC+8)
    摘要: Little is known about the cis-acting elements controlling transcriptional activities of fish rhodopsin and myf-5 genes. Transgenic medaka and zebrafish were used to characterize promoters of carp (Cyprinus carpio) rhodopsin gene (cRh) and zebrafish (Danio rerio) myf-5 gene (myf-5), respectively. Transgenic medaka carrying different lengths of cRh upstream fragments fused with enhanced green fluorescence protein (EGFP) gene revealed several functional regions. Both upstream regions from ?1261 to ?163 bp (?1261/?163) and ?163/?138 contributed to enhance the retina-specific activities of cRh. The cNRE (?75/?63) and CSE (?52/?46) motifs located at the cRh proximal promoter were sufficient to drive the transgene expressed in retinae. The ?73/?68 within cNRE was also proved to be essential for the integrity of the minimal cis-regulatory elements. Regarding the myf-5, transgene expression in zebrafish embryos injected with different deletion fragments of zebrafish myf-5 upstream sequences showed that the ?2776/?2456 and ?1046/?844 regions contributed to the enhancer function. The ?290/?154 region might be involved in controlling the translocation of progenitor muscle cells. Moreover, the upstream ?82/?1 region was identified as a minimal promoter required for somite-specific expression. The ?82/?62 region was the most critical sequence for myf-5 specificity.
    關聯: Marine Technology 4, pp.247-255
    DOI: 10.1007/s10126-002-0016-y
    顯示於類別:[化學學系暨研究所] 期刊論文

    文件中的檔案:

    檔案 描述 大小格式瀏覽次數
    Functional analysis of the proximal promoter regions of fish rhodopsin and myf-5 genes using transgenesis.pdf268KbAdobe PDF0檢視/開啟
    index.html0KbHTML28檢視/開啟

    在機構典藏中所有的資料項目都受到原著作權保護.

    TAIR相關文章

    DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library & TKU Library IR teams. Copyright ©   - 回饋