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    Please use this identifier to cite or link to this item: https://tkuir.lib.tku.edu.tw/dspace/handle/987654321/61854


    Title: Functional analysis of the proximal promoter regions of fish rhodopsin and myf-5 genes using transgenesis
    Authors: 陳曜鴻;Chen, Yau-hung
    Contributors: 淡江大學生命科學研究所
    Date: 2002
    Issue Date: 2011-10-17 00:56:56 (UTC+8)
    Abstract: Little is known about the cis-acting elements controlling transcriptional activities of fish rhodopsin and myf-5 genes. Transgenic medaka and zebrafish were used to characterize promoters of carp (Cyprinus carpio) rhodopsin gene (cRh) and zebrafish (Danio rerio) myf-5 gene (myf-5), respectively. Transgenic medaka carrying different lengths of cRh upstream fragments fused with enhanced green fluorescence protein (EGFP) gene revealed several functional regions. Both upstream regions from ?1261 to ?163 bp (?1261/?163) and ?163/?138 contributed to enhance the retina-specific activities of cRh. The cNRE (?75/?63) and CSE (?52/?46) motifs located at the cRh proximal promoter were sufficient to drive the transgene expressed in retinae. The ?73/?68 within cNRE was also proved to be essential for the integrity of the minimal cis-regulatory elements. Regarding the myf-5, transgene expression in zebrafish embryos injected with different deletion fragments of zebrafish myf-5 upstream sequences showed that the ?2776/?2456 and ?1046/?844 regions contributed to the enhancer function. The ?290/?154 region might be involved in controlling the translocation of progenitor muscle cells. Moreover, the upstream ?82/?1 region was identified as a minimal promoter required for somite-specific expression. The ?82/?62 region was the most critical sequence for myf-5 specificity.
    Relation: Marine Technology 4, pp.247-255
    DOI: 10.1007/s10126-002-0016-y
    Appears in Collections:[Graduate Institute & Department of Chemistry] Journal Article

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